牛体内碳酸酐酶同工酶的选择性组织分布。

Selective tissue distribution of carbonic anhydrase isozymes in the ox.

作者信息

Laurent G, Venot-Giraud N, Marriq C, Limozin N, Garcon D, Filippi D

出版信息

Biochim Biophys Acta. 1977 Mar 15;481(1):222-6. doi: 10.1016/0005-2744(77)90154-1.

Abstract

By affinity chromatography the isozymic distribution of carbonic anhydrase (carbonate hydro-lyase, EC 4.2.1.1) has been studied in extract from various bovine tissues. Carbonic anhydrase II forms isolated from erythrocyte, kidney and brain are indistinguishable by specific activity, amino acid composition, fingerprint, electrophoretic and immunological behaviour. By these criteria they differ from carbonic anhydrase I isolated from rumen epithelium.

摘要

通过亲和层析法，对来自各种牛组织的提取物中碳酸酐酶（碳酸水解酶，EC 4.2.1.1）的同工酶分布进行了研究。从红细胞、肾脏和大脑中分离出的碳酸酐酶II在比活性、氨基酸组成、指纹图谱、电泳和免疫行为方面无法区分。根据这些标准，它们与从瘤胃上皮中分离出的碳酸酐酶I不同。