Badovská Zuzana, Dubayová Katarína, Smolko Lukáš, Toporcerová Silvia, Lukáčová Ivana, Šeršeň Dominika, Mareková Mária, Rabajdová Miroslava
Department of Medical and Clinical Biochemistry, Faculty of Medicine, P. J. Šafárik University, Trieda SNP 1, 040 11, Košice, Slovakia.
Department of Gynaecology and Obstetrics, Faculty of Medicine, P. J. Šafárik University, Trieda SNP 1, 040 11, Košice, Slovakia.
Arch Gynecol Obstet. 2025 Apr 28. doi: 10.1007/s00404-025-08017-3.
In vitro fertilization occurs in a controlled laboratory setting, where oocytes are fertilized by sperm, and the resulting embryos are cultured to the blastocyst stage before transfer to the uterus. The secreted/consumed substances by the embryo in the extracellular environment (secretome) contain a variety of molecules that may provide insights into embryo quality. This study presents new perspectives on the non-invasive and cost-effective assessment and evaluation of embryos during the IVF process, utilizing a spent embryo culture medium (SECM).
The SECM was used from blastocysts prepared for a single blastocyst transfer and was analyzed in two groups-the SECM with successful (F) (n = 30) and unsuccessful (N) (n = 36) embryo implantation in the woman's uterus. Building on our previous next-generation sequencing results, we decided to validate the expression levels of specific miRNAs, particularly hsa-miR-16-5p and hsa-miR-92a-3p, to assess their potential to predict embryo implantation success.
Our results demonstrate different expression levels of miRNA molecules in the monitored groups, which could lead to their use in non-invasive analysis of the implantation potential of embryos in the IVF process. In this study, we employed a metabolomics approach using 3D fluorescence analysis of SECM to identify differences between the studied groups, F and N. Our preliminary results indicate a slightly increased metabolic activity in the group with unsuccessful embryo implantation group.
This is our pilot study where we demonstrated the use of two approaches in analyzing the SECM to predict the implantation potential of embryos in the IVF process which promises further development.
体外受精在可控的实验室环境中进行，卵母细胞在此被精子受精，产生的胚胎在转移至子宫前被培养至囊胚阶段。胚胎在细胞外环境中分泌/消耗的物质（分泌组）包含多种分子，这些分子可能有助于深入了解胚胎质量。本研究利用废弃胚胎培养基（SECM），为体外受精过程中胚胎的非侵入性且经济高效的评估提供了新视角。
使用为单胚胎囊胚移植准备的囊胚的SECM，并将其分为两组进行分析——一组是胚胎在女性子宫内成功着床（F组，n = 30），另一组是胚胎着床失败（N组，n = 36）。基于我们之前的下一代测序结果，我们决定验证特定miRNA，特别是hsa-miR-16-5p和hsa-miR-92a-3p的表达水平，以评估它们预测胚胎着床成功的潜力。
我们的结果表明，监测组中miRNA分子的表达水平不同，这可能使其可用于体外受精过程中胚胎着床潜力的非侵入性分析。在本研究中，我们采用代谢组学方法，通过对SECM进行三维荧光分析来识别F组和N组之间的差异。我们的初步结果表明，胚胎着床失败组的代谢活性略有增加。
这是我们的初步研究，我们展示了使用两种方法分析SECM以预测体外受精过程中胚胎着床潜力，这有望进一步发展。
