Dzhalilova D Sh, Kosyreva A M, Silina M V, Mayak M A, Tsvetkov I S, Makarova O V
Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery, Moscow, Russia.
Bull Exp Biol Med. 2025 Mar;178(5):670-674. doi: 10.1007/s10517-025-06395-x. Epub 2025 Apr 28.
Spontaneous and stimulated by hypoxic conditions and a complex of mitogens (LPS, PHA, and ConA) production of IL-1β and IL-10 by peripheral blood cells of adult male Wistar rats 2 weeks before and 1 month after sublethal hypoxic exposure was examined to determine their tolerance to oxygen deficiency in a decompression chamber. To analyze spontaneous cytokine production, heparinized blood cells were incubated in culture medium for 1 and 24 h (37°C, 21% O and 5% CO). To study cytokine production stimulated by hypoxia, heparinized blood cells were incubated in culture medium for 1 h (37°C, 1% O and 5% CO), and cytokine production stimulated by a complex of mitogens was analyzed by incubating heparinized blood cells in medium supplemented with LPS, PHA, and ConA for 24 h (37°C, 21% O and 5% CO). The levels of proinflammatory cytokine IL-1β and anti-inflammatory cytokine IL-10 in a culture medium were measured by ELISA. The response to hypoxic stimulation and exposure to complex mitogen after sublethal hypoxic exposure in the decompression chamber differs from that before it in high (HR) and low resistant (LR) to hypoxia animals. After hypoxic stimulation and exposure to complex mitogen, the production of proinflammatory cytokine IL-1β increases in both HR and LR rats, but the production of anti-inflammatory IL-10 decreases in LR animals and does not change in HR rats. This indicates the formation of a proinflammatory phenotype in LR animals after sublethal hypoxic exposure. The obtained data should be taken into account when conducting studies using animals with different tolerance to hypoxia.
对成年雄性Wistar大鼠在亚致死性低氧暴露前2周和暴露后1个月，检测其外周血细胞在自发状态下以及在低氧条件和有丝分裂原复合物（脂多糖、植物血凝素和刀豆蛋白A）刺激下白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）的产生情况，以确定它们在减压舱中对缺氧的耐受性。为了分析自发细胞因子的产生，将肝素化的血细胞在培养基中孵育1小时和24小时（37℃，21%氧气和5%二氧化碳）。为了研究低氧刺激下的细胞因子产生，将肝素化的血细胞在培养基中孵育1小时（37℃，1%氧气和5%二氧化碳），通过将肝素化的血细胞在添加脂多糖、植物血凝素和刀豆蛋白A的培养基中孵育24小时（37℃，21%氧气和5%二氧化碳）来分析有丝分裂原复合物刺激下的细胞因子产生。通过酶联免疫吸附测定法测量培养基中促炎细胞因子IL-1β和抗炎细胞因子IL-10的水平。在减压舱中，亚致死性低氧暴露后，高缺氧耐受性（HR）和低缺氧耐受性（LR）动物对低氧刺激和复合有丝分裂原暴露的反应与暴露前不同。低氧刺激和复合有丝分裂原暴露后，HR和LR大鼠中促炎细胞因子IL-1β的产生均增加，但抗炎性IL-10的产生在LR动物中减少，而在HR大鼠中无变化。这表明亚致死性低氧暴露后LR动物中促炎表型的形成。在使用对缺氧耐受性不同的动物进行研究时，应考虑所获得的数据。
