Key Laboratory of the plateau of environmental damage control, Lanzhou General Hospital of Lanzhou Military Command, No. 333 Binhe South Road, Lanzhou, 730050, Gansu, China.
Sci Rep. 2018 Jan 19;8(1):1265. doi: 10.1038/s41598-018-19818-9.
Although extensive studies have focused on the development of acute mountain sickness (AMS), the exact mechanisms of AMS are still obscure. In this study, we used isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis to identify novel AMS-associated biomarkers in human plasma. After 9 hours of hypobaric hypoxia the abundance of proteins related to tricarboxylic acid (TCA) cycle, glycolysis, ribosome, and proteasome were significantly reduced in AMS resistant (AMS-) group, but not in AMS susceptible (AMS+) group. This suggested that AMS- individuals could reduce oxygen consumption via repressing TCA cycle and glycolysis, and reduce energy consumption through decreasing protein degradation and synthesis compared to AMS+ individuals after acute hypoxic exposure. The inflammatory response might be decreased resulting from the repressed TCA cycle. We propose that the ability for oxygen consumption reduction may play an important role in the development of AMS. Our present plasma proteomic study in plateau of the Han Chinese volunteers gives new data to address the development of AMS and potential AMS correlative biomarkers.
尽管已经有大量研究集中在急性高原病 (AMS) 的发展上，但 AMS 的确切机制仍不清楚。在这项研究中，我们使用相对和绝对定量同位素标签 (iTRAQ) 蛋白质组学分析来鉴定人类血浆中与 AMS 相关的新型生物标志物。在低压缺氧 9 小时后，与三羧酸 (TCA) 循环、糖酵解、核糖体和蛋白酶体相关的蛋白质丰度在 AMS 抵抗 (AMS-) 组中显著降低，但在 AMS 敏感 (AMS+) 组中没有降低。这表明与 AMS+个体相比，AMS-个体在急性低氧暴露后可通过抑制 TCA 循环和糖酵解来降低耗氧量，并通过减少蛋白质降解和合成来降低能量消耗。炎症反应可能因 TCA 循环受到抑制而降低。我们提出，降低耗氧量的能力可能在 AMS 的发展中发挥重要作用。我们目前在汉藏族志愿者高原地区的血浆蛋白质组学研究为解决 AMS 的发展和潜在的 AMS 相关生物标志物提供了新的数据。
