Sørensen Anders Nørgaard, Brøndsted Lone
Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg C, Denmark.
Npj Viruses. 2024 Aug 21;2(1):37. doi: 10.1038/s44298-024-00046-0.
The Ackermannviridae family was established in 2017, containing phages previously classified within the Myoviridae family under the Viunalikevirus genus. Ackermannviridae phages have been increasingly studied due to their broad range of hosts among Enterobacteriaceae, and currently, 174 complete genomes are available on NCBI. Instrumental for their wide host infectivity, Ackermannviridae phages display a branched complex of multiple Tail Spike Proteins (TSPs). These TSPs recognize diverse surface polysaccharide receptors, allowing the phages to target strains with distinct lipopolysaccharides or capsular polysaccharides. This review gives an updated overview of the taxonomy and hosts of the expanding Ackermannviridae family with significant emphasis on recent advances in structural and computational biology for elucidating TSP diversity, structural domains, and assembly of the branched TSP complex. Furthermore, we explore the potential of engineering Ackermannviridae phages and discuss the challenges of using transducing wildtype phages for biocontrol. Finally, this review identifies bottlenecks hindering further advances in understanding Ackermannviridae phage biology and applications.
阿克曼病毒科于2017年设立，包含以前归类于肌尾噬菌体科类病毒属的噬菌体。由于阿克曼病毒科噬菌体在肠杆菌科中有广泛的宿主范围，因此对其研究越来越多，目前，美国国立生物技术信息中心（NCBI）上有174个完整基因组。阿克曼病毒科噬菌体具有广泛的宿主感染性，其展示出由多个尾刺蛋白（TSP）组成的分支复合体。这些TSP识别多种表面多糖受体，使噬菌体能够靶向具有不同脂多糖或荚膜多糖的菌株。本综述对不断扩大的阿克曼病毒科的分类和宿主进行了更新概述，重点强调了结构生物学和计算生物学在阐明TSP多样性、结构域和分支TSP复合体组装方面的最新进展。此外，我们探讨了改造阿克曼病毒科噬菌体的潜力，并讨论了使用转导野生型噬菌体进行生物防治的挑战。最后，本综述确定了阻碍阿克曼病毒科噬菌体生物学和应用研究进一步进展的瓶颈。
