Wang MengJie, Pan Yu, Ma Wengjie, Wang Xingrong, Xia Changyou, Wang Yue
State Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
College of Veterinary Medicine, Southwest University, Chongqing, 400715, China.
BMC Vet Res. 2025 Apr 29;21(1):297. doi: 10.1186/s12917-025-04688-x.
Porcine respiratory disease complex poses a substantial economic challenge for the swine farms due to its prevalence in pig farming environments. The disease is primarily caused by viral pathogens such as swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus type 2 (PCV2). Given the high incidence and morbidity associated with these viruses, effective control strategies rely on rapid and accurate diagnosis.
To this end, we developed and validated a triplex TaqMan probe-based real-time PCR assay for the simultaneous detection and differentiation of SIV, PRRSV, and PCV2. This assay demonstrated high specificity, with no observed cross-reactivity between the target viruses. The assay's sensitivity was determined to be 100 copies/µL for SIV and 10 copies/µL for PRRSV and PCV2. Comparison with a national standard detection method using 110 clinical samples revealed 100% agreement, confirming the reliability of the newly developed assay for clinical diagnostic applications.
Given the zoonotic potential of SIV, this assay not only provides a valuable diagnostic tool for veterinary medicine but also contributes to enhanced public health surveillance efforts.
猪呼吸道疾病综合征因其在养猪环境中的普遍存在，给猪场带来了巨大的经济挑战。该疾病主要由猪流感病毒（SIV）、猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV2）等病毒病原体引起。鉴于这些病毒的高发病率和高致病性，有效的控制策略依赖于快速准确的诊断。
为此，我们开发并验证了一种基于三重TaqMan探针的实时荧光定量PCR检测方法，用于同时检测和鉴别SIV、PRRSV和PCV2。该检测方法具有高度特异性，目标病毒之间未观察到交叉反应。该检测方法对SIV的灵敏度测定为100拷贝/微升，对PRRSV和PCV2的灵敏度为10拷贝/微升。使用110份临床样本与国家标准检测方法进行比较，结果显示一致性为100%，证实了新开发的检测方法在临床诊断应用中的可靠性。
鉴于SIV的人畜共患病潜力，该检测方法不仅为兽医学提供了一种有价值的诊断工具，也有助于加强公共卫生监测工作。
