Signorini Cinzia, Corsaro Roberta, Collodel Giulia, Maettner Robert, Sterzik Karl, Strehler Erwin, Liguori Laura, Moretti Elena
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
MVZ Next Fertility Ulm GmbH, 89077 Ulm, Germany.
Antioxidants (Basel). 2025 Mar 25;14(4):382. doi: 10.3390/antiox14040382.
This study evaluated the effects of chlorogenic acid (CGA) on human semen and on oxidative stress (OS) induced in vitro in human spermatozoa. After the treatment of the basal semen with 100 µM CGA, rapid and slow sperm progressive motility were evaluated and seminal F-Isoprostanes (F-IsoPs), a marker of OS, were quantified by ELISA. In a second set of experiments, semen was treated with 100 µM CGA, 1 mM HO to induce OS, or HO+CGA; untreated samples were used as controls. Then, sperm motility, DNA integrity by the acridine orange test, F-IsoPs and mRNA expression by RT-PCR were quantified. In CGA-treated specimens, rapid progressive sperm motility was increased ( < 0.01) and F-IsoP levels decreased ( < 0.001) versus controls. The increase of F-IsoP levels and DNA damage and the decrease of sperm motility after HO treatment was reversed in the presence of CGA, which upregulated mRNA expression. These findings contributed to clarifying CGA's antioxidant activity and highlighted the positive impact of CGA on sperm progressive motility, suggesting also a possible mechanism of action based on the Nrf2 pathway. CGA can be useful during human semen handling procedures in the laboratory and in optimizing the recovery of motile spermatozoa through selection techniques during assisted reproductive technology protocols.
本研究评估了绿原酸（CGA）对人类精液以及体外诱导人类精子产生的氧化应激（OS）的影响。用100µM CGA处理基础精液后，评估快速和慢速精子前向运动能力，并通过酶联免疫吸附测定法（ELISA）对精液中氧化应激标志物F-异前列腺素（F-IsoPs）进行定量分析。在第二组实验中，精液分别用100µM CGA、1mM过氧化氢（HO）以诱导氧化应激或HO+CGA进行处理；未处理的样本用作对照。然后，对精子运动能力、通过吖啶橙试验检测的DNA完整性、F-IsoPs以及通过逆转录聚合酶链反应（RT-PCR）检测的mRNA表达进行定量分析。与对照组相比，在CGA处理的样本中，快速前向精子运动能力增强（<0.01），F-IsoP水平降低（<0.001）。在CGA存在的情况下，HO处理后F-IsoP水平的升高、DNA损伤以及精子运动能力的降低均得到逆转，同时CGA上调了mRNA表达。这些发现有助于阐明CGA的抗氧化活性，并突出了CGA对精子前向运动能力的积极影响，还提出了一种基于核因子E2相关因子2（Nrf2）途径的可能作用机制。CGA在实验室人类精液处理过程中以及在辅助生殖技术方案中通过选择技术优化活动精子的回收率方面可能会有所帮助。
