Belechheb Taoufik, Yemalahi Anass, Bouhnik Omar, Hassani Mounir Zerrouk, El Galiou Ouiam, Laglaoui Amin, Bakkali Mohammed, El Idrissi Mustapha Missbah, Arakrak Abdelhay
Équipe de Recherche en Biotechnologies and Génie des Biomolécules, Faculté des Sciences et Techniques de Tanger, Université Abdelmalek Essaadi, B.P.: 416, Tangier, Morocco.
Laboratory of Biotechnology, Environment, Agrifood, and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P 1796, 30050, Fez, Morocco.
Curr Microbiol. 2025 Apr 29;82(6):267. doi: 10.1007/s00284-025-04249-3.
Genista monspessulana is a wild legume of high fodder value in northern Morocco, where it contributes to livestock feeding, particularly during the lean season. The plant fixes nitrogen in symbiosis with soil bacteria known as rhizobia. To identify and characterize its symbiotic partners, we isolated twenty-two bacteria inhabiting the plant nodules and assessed their phenotypic and genetic diversity as well as their symbiotic efficiency. The 16S rRNA sequences analysis proved that 7 isolates were affiliated with the genus Bradyrhizobium, which significantly improve plant growth under nitrogen deficiency. Based on multi-locus sequence analysis using five different housekeeping genes, three representative strains were selected for further analyses. The phylogenetic analysis of the concatenated sequences of the five genes showed that the closest type strain is Bradyrhizobium canariense LMG 2122265T. The strains nodulate also other Genisteae such as Cytisus villosus and Lupinus luteus besides their host plant. The phylogenetic analysis of the symbiotic nodC gene allowed the assignment of the strains to the symbiovar genistearum. The three strains proved to be very efficient in the fixation of N as revealed by their relative and absolute efficiency indexes and may be used as effective individual or mixed inocula, to improve the plant growth in its natural habitat and contribute to soil restoration, and revegetation in Northern Morocco.
滨海金雀儿是摩洛哥北部一种具有高饲料价值的野生豆科植物，它对牲畜饲养有重要作用，尤其是在青黄不接的季节。该植物与被称为根瘤菌的土壤细菌共生固氮。为了鉴定和表征其共生伙伴，我们从植物根瘤中分离出22种细菌，并评估了它们的表型和遗传多样性以及共生效率。16S rRNA序列分析证明，7个分离株属于慢生根瘤菌属，该属在缺氮条件下能显著促进植物生长。基于使用五个不同管家基因的多位点序列分析，选择了三个代表性菌株进行进一步分析。对这五个基因的串联序列进行系统发育分析表明，最接近的模式菌株是加那利慢生根瘤菌LMG 2122265T。除了其寄主植物外，这些菌株还能使其他金雀儿属植物如绒毛金雀儿和黄羽扇豆结瘤。对共生结瘤基因nodC的系统发育分析使这些菌株被归入共生型金雀儿共生型。通过相对和绝对效率指标显示，这三个菌株在固氮方面非常高效，可作为有效的单独或混合接种剂，用于改善其天然栖息地的植物生长，并有助于摩洛哥北部的土壤修复和植被恢复。
