Yang Kaixin, Wei Wenping, Xie Xiaoman, Zhang Haoran, Li Huanhuan, Wang Pengbo, Wang Kun, Dai Shuhan, Liu Ying, Zheng Wanlin, Xu Li, Yan Jinyong, Yang Min, Yan Yunjun
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
ACS Appl Mater Interfaces. 2025 May 14;17(19):28327-28335. doi: 10.1021/acsami.5c07284. Epub 2025 Apr 29.
Photosynthetic semiconductor biohybrids, which combine the light-harvesting capacity of semiconductors and catalytic activity of whole-cell microorganisms, show substantial potential for advancing bioremediation technology. However, few yeast-based biohybrid systems for pollutant removal were reported. In this study, we have constructed a whole-cell biohybrid system based on featuring synthesized biocompatible cadmium sulfide (CdS) nanoparticles (NPs) for the photocatalytic reduction of hexavalent chromium [Cr(VI)] under UV irradiation. The integration of these CdS NPs onto the surface of modified cells endowed the system with excellent photocatalytic performance, achieving 100% Cr(VI) reduction within 2 h. The system exhibited a higher kinetic constant (0.03 min). In the trapping experiments, the reactive oxygen species (ROS) generated photochemically, specifically the superoxide anion (•O), which were identified as crucial mediators that facilitate the reduction of Cr(VI). The enhanced activity of the -CdS biohybrid was attributed to efficient electron transfer. Additionally, through transcriptome analysis, we found that the differentially expressed genes are associated with membrane transport, oxidation-reduction process, energy metabolism, and electron transfer. This whole-cell biohybrid catalytic strategy holds promise as an innovative approach for the reduction of Cr(VI) and has the potential to enhance our understanding of the interactions among light, inorganic material, and microorganisms.
光合半导体生物杂交体结合了半导体的光捕获能力和全细胞微生物的催化活性，在推进生物修复技术方面显示出巨大潜力。然而，报道的用于污染物去除的基于酵母的生物杂交系统很少。在本研究中，我们构建了一种基于合成生物相容性硫化镉（CdS）纳米颗粒（NPs）的全细胞生物杂交系统，用于在紫外线照射下光催化还原六价铬[Cr(VI)]。将这些CdS NPs整合到修饰细胞表面赋予了该系统优异的光催化性能，在2小时内实现了100%的Cr(VI)还原。该系统表现出更高的动力学常数（0.03 min）。在捕获实验中，光化学产生的活性氧（ROS），特别是超氧阴离子（•O），被确定为促进Cr(VI)还原的关键介质。-CdS生物杂交体活性的增强归因于有效的电子转移。此外，通过转录组分析，我们发现差异表达基因与膜运输、氧化还原过程、能量代谢和电子转移有关。这种全细胞生物杂交催化策略有望成为还原Cr(VI)的创新方法，并有潜力增进我们对光、无机材料和微生物之间相互作用的理解。
