Kang CongBao
Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A∗STAR), 10 Biopolis Road, #05-01, 138670, Singapore.
Eur J Med Chem. 2025 Aug 5;292:117682. doi: 10.1016/j.ejmech.2025.117682. Epub 2025 Apr 23.
RNA molecules play essential roles in numerous biological pathways, making them attractive targets for drug discovery. Despite the challenges in developing small molecules targeting RNA, the success in developing compounds that modulate RNA function underscores its therapeutic potential. F NMR spectroscopy has emerged as a powerful tool in structural biology and drug discovery, particularly for studying macromolecular structures and ligand interactions. As RNA continues to gain prominence as a drug target, F NMR is expected to play a pivotal role in advancing RNA-focused drug discovery. This review describes the diverse applications of F NMR in RNA biology, including its use in characterizing RNA structures, probing molecular dynamics, identifying small-molecule binders, and investigating interaction mechanisms of small-molecule ligands. By providing detailed structural and ligand binding insights, F NMR will facilitate the discovery of RNA-targeting therapeutics and deepen our understanding of RNA modulatory mechanisms.
RNA分子在众多生物途径中发挥着重要作用，这使其成为药物研发的有吸引力的靶点。尽管开发靶向RNA的小分子存在挑战，但开发调节RNA功能的化合物的成功突出了其治疗潜力。氟核磁共振光谱已成为结构生物学和药物研发中的一种强大工具，特别是用于研究大分子结构和配体相互作用。随着RNA作为药物靶点的地位不断凸显，氟核磁共振有望在推进以RNA为重点的药物研发中发挥关键作用。本综述描述了氟核磁共振在RNA生物学中的多种应用，包括其在表征RNA结构、探究分子动力学、鉴定小分子结合剂以及研究小分子配体的相互作用机制方面的应用。通过提供详细的结构和配体结合见解，氟核磁共振将促进靶向RNA疗法的发现，并加深我们对RNA调节机制的理解。
