• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鸭瘟病毒 UL14 基因编码的蛋白调节病毒形态发生，并影响病毒复制。

The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China.

出版信息

Poult Sci. 2022 Jun;101(6):101863. doi: 10.1016/j.psj.2022.101863. Epub 2022 Mar 17.

DOI:10.1016/j.psj.2022.101863
PMID:35489250
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9062478/
Abstract

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.

摘要

为了研究鸭瘟病毒 (DPV) 被膜蛋白 UL14 在病毒复制中的关键作用，我们利用细菌人工染色体系统生成了 2 种 DPV 突变病毒，即 UL14 缺失突变病毒 (CHv-BAC-ΔUL14) 和相应的回复突变病毒 (CHv-BAC-ΔUL14R)。我们发现，CHv-BAC-ΔUL14 病毒在透射电子显微镜 (TEM) 研究中表现出病毒粒子形态发生受损。此外，CHv-BAC-ΔUL14 在鸭胚成纤维细胞 (DEF) 中表现出蚀斑大小减小。最后，CHv-BAC-ΔUL14 表现出明显的病毒生长缺陷。综上所述，我们的研究结果表明，DPV UL14 蛋白调节病毒形态发生以实现有效的病毒复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/fc1500766d80/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/c5deaf4856e9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/913dfd912293/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/0596c4d2a15d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/79d74b94c7b1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/12614c95e675/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/fc1500766d80/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/c5deaf4856e9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/913dfd912293/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/0596c4d2a15d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/79d74b94c7b1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/12614c95e675/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b082/9062478/fc1500766d80/gr6.jpg

相似文献

1
The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication.鸭瘟病毒 UL14 基因编码的蛋白调节病毒形态发生，并影响病毒复制。
Poult Sci. 2022 Jun;101(6):101863. doi: 10.1016/j.psj.2022.101863. Epub 2022 Mar 17.
2
Duck plague virus gE serves essential functions during the virion final envelopment through influence capsids budding into the cytoplasmic vesicles.鸭瘟病毒 gE 通过影响衣壳进入细胞质小泡出芽在病毒粒子最终包膜中发挥重要功能。
Sci Rep. 2020 Mar 27;10(1):5658. doi: 10.1038/s41598-020-62604-9.
3
The Pivotal Roles of US3 Protein in Cell-to-Cell Spread and Virion Nuclear Egress of Duck Plague Virus.鸭瘟病毒 US3 蛋白在细胞间传播和病毒核输出中的关键作用。
Sci Rep. 2020 Apr 28;10(1):7181. doi: 10.1038/s41598-020-64190-2.
4
Duck plague virus Glycoprotein J is functional but slightly impaired in viral replication and cell-to-cell spread.鸭瘟病毒糖蛋白 J 具有功能，但在病毒复制和细胞间传播方面略有缺陷。
Sci Rep. 2018 Mar 6;8(1):4069. doi: 10.1038/s41598-018-22447-x.
5
Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone.基于感染性中国强毒株鸭肠炎病毒细菌人工染色体克隆的UL55基因初步研究
Virol J. 2017 Apr 13;14(1):78. doi: 10.1186/s12985-017-0748-y.
6
Evaluation of the Safety and Immunogenicity of Duck-Plague Virus Mutants.鸭瘟病毒突变株的安全性和免疫原性评估。
Front Immunol. 2022 Apr 20;13:882796. doi: 10.3389/fimmu.2022.882796. eCollection 2022.
7
Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication.研究报告：鸭瘟病毒 pUL48 是一种晚期蛋白，在病毒复制过程中发挥着重要作用。
Poult Sci. 2023 Feb;102(2):102358. doi: 10.1016/j.psj.2022.102358. Epub 2022 Nov 19.
8
Research Note: Duck plague virus glycoprotein I influences cell-cell spread and final envelope acquisition.研究报告：鸭瘟病毒糖蛋白 I 影响细胞间传播和最终包膜获得。
Poult Sci. 2020 Dec;99(12):6647-6652. doi: 10.1016/j.psj.2020.08.065. Epub 2020 Sep 15.
9
US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.US10 蛋白对鸭肠炎病毒体外感染至关重要但非必需。
Sci Rep. 2018 Nov 7;8(1):16510. doi: 10.1038/s41598-018-34503-7.
10
Duck plague virus-encoded microRNA dev-miR-D28-3p inhibits viral replication via targeting UL27.鸭瘟病毒编码的 microRNA 病毒 miR-D28-3p 通过靶向 UL27 抑制病毒复制。
Vet Microbiol. 2024 Oct;297:110202. doi: 10.1016/j.vetmic.2024.110202. Epub 2024 Jul 29.

引用本文的文献

1
Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ.强毒株鸭肠炎病毒XJ株的全基因组序列测定及感染性细菌人工染色体克隆构建
Transbound Emerg Dis. 2024 May 17;2024:1746963. doi: 10.1155/2024/1746963. eCollection 2024.
2
Identification and subcellular localization of proteins that interact with Duck plague virus pUL14 in infected host cells.感染宿主细胞中与鸭瘟病毒pUL14相互作用的蛋白质的鉴定及亚细胞定位
Poult Sci. 2025 Jan;104(1):104649. doi: 10.1016/j.psj.2024.104649. Epub 2024 Dec 9.
3
Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro.

本文引用的文献

1
DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication.DPV UL41 基因编码的蛋白诱导宿主关闭活性并影响病毒复制。
Vet Microbiol. 2021 Apr;255:108979. doi: 10.1016/j.vetmic.2021.108979. Epub 2021 Feb 27.
2
The Pivotal Roles of US3 Protein in Cell-to-Cell Spread and Virion Nuclear Egress of Duck Plague Virus.鸭瘟病毒 US3 蛋白在细胞间传播和病毒核输出中的关键作用。
Sci Rep. 2020 Apr 28;10(1):7181. doi: 10.1038/s41598-020-64190-2.
3
Duck plague virus gE serves essential functions during the virion final envelopment through influence capsids budding into the cytoplasmic vesicles.
鸭肠炎病毒LORF4基因是一个晚期基因，对病毒在体外的复制并非必需。
Poult Sci. 2024 Dec;103(12):104275. doi: 10.1016/j.psj.2024.104275. Epub 2024 Aug 31.
4
The precise function of alphaherpesvirus tegument proteins and their interactions during the viral life cycle.甲型疱疹病毒被膜蛋白在病毒生命周期中的精确功能及其相互作用。
Front Microbiol. 2024 Jul 2;15:1431672. doi: 10.3389/fmicb.2024.1431672. eCollection 2024.
鸭瘟病毒 gE 通过影响衣壳进入细胞质小泡出芽在病毒粒子最终包膜中发挥重要功能。
Sci Rep. 2020 Mar 27;10(1):5658. doi: 10.1038/s41598-020-62604-9.
4
US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.US10 蛋白对鸭肠炎病毒体外感染至关重要但非必需。
Sci Rep. 2018 Nov 7;8(1):16510. doi: 10.1038/s41598-018-34503-7.
5
Visualizing the Essential Role of Complete Virion Assembly Machinery in Efficient Hepatitis C Virus Cell-to-Cell Transmission by a Viral Infection-Activated Split-Intein-Mediated Reporter System.通过病毒感染激活的分裂内含肽介导的报告系统，可视化完整病毒体组装机制在丙型肝炎病毒细胞间高效传播中的重要作用。
J Virol. 2017 Jan 3;91(2). doi: 10.1128/JVI.01720-16. Print 2017 Jan 15.
6
The Interaction between Herpes Simplex Virus 1 Tegument Proteins UL51 and UL14 and Its Role in Virion Morphogenesis.单纯疱疹病毒1型被膜蛋白UL51和UL14之间的相互作用及其在病毒粒子形态发生中的作用。
J Virol. 2016 Sep 12;90(19):8754-67. doi: 10.1128/JVI.01258-16. Print 2016 Oct 1.
7
Comparative genomic analysis of duck enteritis virus strains.鸭肠炎病毒株的比较基因组分析。
J Virol. 2012 Dec;86(24):13841-2. doi: 10.1128/JVI.01517-12.
8
Complete genomic sequence of Chinese virulent duck enteritis virus.中国强毒鸭肠炎病毒的全基因组序列。
J Virol. 2012 May;86(10):5965. doi: 10.1128/JVI.00529-12.
9
Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16.单纯疱疹病毒 1 型 UL14 衣壳蛋白调节主要衣壳蛋白 VP16 的细胞内区室化。
Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.
10
Virus cell-to-cell transmission.病毒细胞间传播。
J Virol. 2010 Sep;84(17):8360-8. doi: 10.1128/JVI.00443-10. Epub 2010 Apr 7.