鸭瘟病毒 UL14 基因编码的蛋白调节病毒形态发生，并影响病毒复制。

The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China.

出版信息

Poult Sci. 2022 Jun;101(6):101863. doi: 10.1016/j.psj.2022.101863. Epub 2022 Mar 17.

DOI:10.1016/j.psj.2022.101863

PMID:35489250

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9062478/

Abstract

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.

摘要

为了研究鸭瘟病毒 (DPV) 被膜蛋白 UL14 在病毒复制中的关键作用，我们利用细菌人工染色体系统生成了 2 种 DPV 突变病毒，即 UL14 缺失突变病毒 (CHv-BAC-ΔUL14) 和相应的回复突变病毒 (CHv-BAC-ΔUL14R)。我们发现，CHv-BAC-ΔUL14 病毒在透射电子显微镜 (TEM) 研究中表现出病毒粒子形态发生受损。此外，CHv-BAC-ΔUL14 在鸭胚成纤维细胞 (DEF) 中表现出蚀斑大小减小。最后，CHv-BAC-ΔUL14 表现出明显的病毒生长缺陷。综上所述，我们的研究结果表明，DPV UL14 蛋白调节病毒形态发生以实现有效的病毒复制。