Detection and Analysis of Drug and Disinfectant Resistance Genes in the Sewage of a Center for Disease Control and Prevention.

PURPOSE

Sewage is a significant reservoir for drug and disinfectant resistance genes and a medium for dissemination. This study aimed to evaluate the presence of drug and disinfectant resistance genes in the sewage of a Center for Disease Control and Prevention (CDC) and to assess the risks of their dissemination.

METHODS

Sewage from a CDC in Hangzhou was collected, filtered, and enriched, and its microorganisms were cultured. The isolated bacteria were identified, and the minimum inhibitory concentration (MIC) was determined. The drug and disinfectant resistance genes in the sewage and bacteria were detected through polymerase chain reaction amplification.

RESULTS

Three kinds of bacteria were isolated from the sewage sample. The MIC for and against chlorine-containing disinfectants was 250 mg/L, whereas the MIC for was 500 mg/L. The β-lactam resistance gene and the disinfectant resistance gene were positive in the bacteria, whereas the β-lactam resistance genes , and , the tetracycline resistance gene , the aminoglycoside resistance genes and , and the disinfectant resistance genes , and were positive in the sewage.

CONCLUSION

Drug and disinfectant resistance genes were found in the sewage of a CDC and were associated with bacteria. Thus, optimizing the monitoring and treatment of sewage is crucial.