Khaleghi Khorrami Alireza, Shokri Rasoul, Athari Seyyed Shamsadin, Mahmazi Sanaz
Department of Microbiology, ZA. C., Islamic Azad University, Zanjan, Iran.
Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Adv Biomed Res. 2025 Mar 28;14:28. doi: 10.4103/abr.abr_570_24. eCollection 2025.
The study conclusively demonstrated that () neutrophil-activating protein (HP-NAP) has the ability to switch a pro-allergic Th2 response to a Th1 response. Furthermore, it investigated the effects of HP-NAP on TH1 and TH2 cytokines, as well as airway inflammation in a rat model of allergic asthma, and compared these effects with those of .
and HP-NAP were prepared based on a previous study. Sixty male Blab/c mice were used for histopathological and immunological studies, divided into six groups of 10. Five mice from each group were selected for histopathological studies, and five were selected for measuring changes in TH cytokine levels and eosinophil count. The study was conducted over 31 days.
Histological staining was performed on six selected groups. After treatment with HP-NAP, the recipient treatment group demonstrated a significant decrease in interleukin (IL)-4, IL-5, and total immunoglobulin E (IgE) levels, along with a significant increase in IL-2 and interferon-gamma (IFN-γ) levels. Furthermore, the number of eosinophils in the bronchoalveolar lavage fluid (BALF) of mice treated with HP-NAP significantly decreased compared to the positive control samples.
Our research findings conclude that HP-NAP can significantly reduce serum levels of IgE, IL-5, and IL-4, while increasing levels of IL-2 and IFN-γ, compared to . HP-NAP can be a useful therapeutic agent for preventing and treating allergic asthma.
该研究确凿地证明，幽门螺杆菌中性粒细胞激活蛋白（HP-NAP）有能力将促过敏性Th2反应转变为Th1反应。此外，该研究还调查了HP-NAP对过敏性哮喘大鼠模型中Th1和Th2细胞因子以及气道炎症的影响，并将这些影响与[具体物质]的影响进行了比较。
[具体物质]和HP-NAP根据先前的研究制备。60只雄性BALB/c小鼠用于组织病理学和免疫学研究，分为6组，每组10只。每组选取5只小鼠进行组织病理学研究，5只用于测量Th细胞因子水平和嗜酸性粒细胞计数的变化。该研究持续31天。
对6个选定组进行了组织学染色。用HP-NAP治疗后，受体治疗组的白细胞介素（IL）-4、IL-5和总免疫球蛋白E（IgE）水平显著降低，同时IL-2和干扰素-γ（IFN-γ）水平显著升高。此外，与阳性对照样本相比，用HP-NAP治疗的小鼠支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞数量显著减少。
我们的研究结果表明，与[具体物质]相比，HP-NAP可显著降低血清IgE、IL-5和IL-4水平，同时提高IL-2和IFN-γ水平。HP-NAP可能是预防和治疗过敏性哮喘的一种有用的治疗剂。
