• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

中国一株天然基因缺失的猪传染性胃肠炎病毒的致病性鉴定与评价

Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China.

作者信息

Xu Lei, Dai Hong-Bo, Luo Zhi-Peng, Zhu Ling, Zhao Jun, Lee Feng-Qin, Liu Ze-Yan, Nie Min-Cai, Wang Xue-Tao, Zhou Yuan-Cheng, Xu Zhi-Wen

机构信息

College of Veterinary Medicine, Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Veterinary Biologicals Engineering and Technology Research Center of Sichuan Province, Animtech Bioengineering Co. Ltd., Chengdu 610066, China.

出版信息

Transbound Emerg Dis. 2023 Mar 3;2023:2652850. doi: 10.1155/2023/2652850. eCollection 2023.

DOI:10.1155/2023/2652850
PMID:40303681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12017154/
Abstract

Porcine transmissible gastroenteritis virus is the major pathogen that causes fatal diarrhea in newborn piglets. In this study, a TGEV strain was isolated from the small intestine of diarrhea piglets in Sichuan Province, China, and designated SC2021. The complete genomic sequence of TGEV SC2021 was 28561 bp, revealing a new natural deletion TGEV strain. Based on phylogenetic analyses, TGEV SC2021 belonged to the Miller cluster and was closely related to CN strains. The newborn piglets orally challenged with TGEV SC2021 showed typical watery diarrhea. In addition, macro and micropathological changes in the lungs and intestines were observed. In conclusion, we isolated a new natural deletion virus strain and confirmed that the virus strain has high pathogenicity in newborn piglets. Moreover, macroscopic and microscopic lesions were observed in the lungs and intestines of all TGEV SC2021-infected piglets. In summary, we isolated a new natural deletion TGEV strain and demonstrated that the natural deletion strain showed high pathogenicity in newborn piglets. These data enrich the diversity of TGEV strains and help us to understand the genetic evolution and molecular pathogenesis of TGEV.

摘要

猪传染性胃肠炎病毒是导致新生仔猪致命性腹泻的主要病原体。在本研究中，从中国四川省腹泻仔猪的小肠中分离出一株猪传染性胃肠炎病毒，命名为SC2021。猪传染性胃肠炎病毒SC2021的全基因组序列为28561bp，揭示了一种新的自然缺失型猪传染性胃肠炎病毒株。基于系统发育分析，猪传染性胃肠炎病毒SC2021属于Miller簇，与CN株密切相关。用猪传染性胃肠炎病毒SC2021口服攻毒的新生仔猪表现出典型的水样腹泻。此外，还观察到肺和肠道的大体和微观病理变化。总之，我们分离出一种新的自然缺失病毒株，并证实该病毒株对新生仔猪具有高致病性。此外，在所有感染猪传染性胃肠炎病毒SC2021的仔猪的肺和肠道中均观察到宏观和微观病变。综上所述，我们分离出一种新的自然缺失型猪传染性胃肠炎病毒株，并证明该自然缺失株对新生仔猪具有高致病性。这些数据丰富了猪传染性胃肠炎病毒株的多样性，有助于我们了解猪传染性胃肠炎病毒的遗传进化和分子发病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/49b124baa5ac/TBED2023-2652850.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/b36da437025f/TBED2023-2652850.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/03b66268db7f/TBED2023-2652850.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/824c84e6aa02/TBED2023-2652850.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/92c805d7e060/TBED2023-2652850.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/49b124baa5ac/TBED2023-2652850.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/b36da437025f/TBED2023-2652850.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/03b66268db7f/TBED2023-2652850.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/824c84e6aa02/TBED2023-2652850.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/92c805d7e060/TBED2023-2652850.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef7/12017154/49b124baa5ac/TBED2023-2652850.005.jpg

相似文献

1
Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China.中国一株天然基因缺失的猪传染性胃肠炎病毒的致病性鉴定与评价
Transbound Emerg Dis. 2023 Mar 3;2023:2652850. doi: 10.1155/2023/2652850. eCollection 2023.
2
Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China.中国一株自然重组传染性胃肠炎病毒的特性分析与致病性评估。
Virology. 2020 Jun;545:24-32. doi: 10.1016/j.virol.2020.03.001. Epub 2020 Mar 6.
3
Identification of a natural recombinant transmissible gastroenteritis virus between Purdue and Miller clusters in China.中国普渡株和米勒株之间天然重组传染性胃肠炎病毒的鉴定
Emerg Microbes Infect. 2017 Aug 23;6(8):e74. doi: 10.1038/emi.2017.62.
4
Molecular and phylogenetic analysis of transmissible gastroenteritis virus strain VET-16, isolated from piglets in Vietnam.分子和系统进化分析从越南仔猪分离的传染性胃肠炎病毒株 VET-16。
Arch Virol. 2024 Aug 21;169(9):183. doi: 10.1007/s00705-024-06101-8.
5
ORF3a deletion in field strains of porcine-transmissible gastroenteritis virus in China: A hint of association with porcine respiratory coronavirus.中国猪传染性胃肠炎病毒田间毒株的ORF3a缺失：与猪呼吸道冠状病毒关联的线索
Transbound Emerg Dis. 2017 Jun;64(3):698-702. doi: 10.1111/tbed.12634. Epub 2017 Mar 15.
6
Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China.从中国分离的猪传染性胃肠炎病毒HX株的分子特征及系统发育分析
BMC Vet Res. 2015 Mar 21;11:72. doi: 10.1186/s12917-015-0387-8.
7
Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd.在一个猪群中共同传播的传染性胃肠炎冠状病毒（TGEV）和猪呼吸道冠状病毒（PRCV）田间分离株的分子特征与发病机制
Arch Virol. 2000;145(6):1133-47. doi: 10.1007/s007050070114.
8
Transmissible Gastroenteritis Virus (TGEV) and Porcine Respiratory Coronavirus (PRCV): Epidemiology and Molecular Characteristics-An Updated Overview.传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）：流行病学与分子特征——最新综述
Viruses. 2025 Mar 28;17(4):493. doi: 10.3390/v17040493.
9
The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets.刺突蛋白 N 端结构域不是猪传染性胃肠炎病毒在仔猪中肠嗜性的决定因素。
Viruses. 2019 Mar 30;11(4):313. doi: 10.3390/v11040313.
10
Persistent Transmissible Gastroenteritis Virus Infection Enhances Enterotoxigenic Escherichia coli K88 Adhesion by Promoting Epithelial-Mesenchymal Transition in Intestinal Epithelial Cells.持续性传染性胃肠炎病毒感染通过促进肠上皮细胞的上皮-间质转化增强产肠毒素大肠杆菌K88的黏附。
J Virol. 2017 Oct 13;91(21). doi: 10.1128/JVI.01256-17. Print 2017 Nov 1.

引用本文的文献

1
Binding of transmissible gastroenteritis virus and porcine respiratory coronavirus to human and porcine aminopeptidase N receptors as an indicator of cross-species transmission.传染性胃肠炎病毒和猪呼吸道冠状病毒与人及猪氨肽酶N受体的结合作为跨物种传播的指标
PLoS One. 2025 May 27;20(5):e0325023. doi: 10.1371/journal.pone.0325023. eCollection 2025.

本文引用的文献

1
Porcine Respiratory Coronavirus as a Model for Acute Respiratory Coronavirus Disease.猪呼吸冠状病毒作为急性呼吸道冠状病毒病的模型。
Front Immunol. 2022 Mar 28;13:867707. doi: 10.3389/fimmu.2022.867707. eCollection 2022.
2
The Immunity Protection of Central Nervous System Induced by Pseudorabies Virus DelgI/gE/TK in Mice.伪狂犬病病毒缺失gI/gE/TK株对小鼠中枢神经系统的免疫保护作用
Front Microbiol. 2022 Mar 25;13:862907. doi: 10.3389/fmicb.2022.862907. eCollection 2022.
3
Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China.
中国东北地区一株猪传染性胃肠炎冠状病毒的分离与鉴定
Front Vet Sci. 2021 Mar 2;8:611721. doi: 10.3389/fvets.2021.611721. eCollection 2021.
4
Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China.中国一株自然重组传染性胃肠炎病毒的特性分析与致病性评估。
Virology. 2020 Jun;545:24-32. doi: 10.1016/j.virol.2020.03.001. Epub 2020 Mar 6.
5
The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV).利用从 ANPEP 敲除猪中提取的细胞来评估氨肽酶 N（APN）作为猪德尔塔冠状病毒（PDCoV）受体的作用。
Virology. 2020 Feb;541:136-140. doi: 10.1016/j.virol.2019.12.007. Epub 2019 Dec 24.
6
Decline of transmissible gastroenteritis virus and its complex evolutionary relationship with porcine respiratory coronavirus in the United States.美国传染性胃肠炎病毒的衰减及其与猪呼吸冠状病毒的复杂进化关系。
Sci Rep. 2019 Mar 8;9(1):3953. doi: 10.1038/s41598-019-40564-z.
7
Case Report of Transmissible Gastroenteritis Coronavirus Infection Associated with Small Intestine and Brain Lesions in Piglets.
Viral Immunol. 2019 Jan/Feb;32(1):63-67. doi: 10.1089/vim.2018.0095. Epub 2018 Sep 12.
8
Retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern China.中国南方猪群中猪急性腹泻综合征冠状病毒的回溯性检测与系统进化分析。
Transbound Emerg Dis. 2019 Mar;66(2):687-695. doi: 10.1111/tbed.13008. Epub 2019 Jan 9.
9
Identification of a natural recombinant transmissible gastroenteritis virus between Purdue and Miller clusters in China.中国普渡株和米勒株之间天然重组传染性胃肠炎病毒的鉴定
Emerg Microbes Infect. 2017 Aug 23;6(8):e74. doi: 10.1038/emi.2017.62.
10
A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge.一种活的gI/gE缺失伪狂犬病病毒（PRV）可保护断奶仔猪免受致死性变异PRV的攻击。
Virus Genes. 2017 Aug;53(4):565-572. doi: 10.1007/s11262-017-1454-y. Epub 2017 Apr 17.