中国一株自然重组传染性胃肠炎病毒的特性分析与致病性评估。

Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China.

机构信息

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China.

出版信息

Virology. 2020 Jun;545:24-32. doi: 10.1016/j.virol.2020.03.001. Epub 2020 Mar 6.

Porcine transmissible gastroenteritis virus (TGEV) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. In this study, a TGEV JS2012 strain was isolated from the feces of piglets in Jiangsu Province, China. The phylogenetic analysis showed that TGEV JS2012 was placed between the Purdue and the Miller clusters. Analysis of recombination confirmed that TGEV JS2012 is a natural recombinant strain between Miller M6 and Purdue 115. Similar to Miller M6, virulent Purdue and China strain TS, in S gene the JS2012 maintained genetic integrity and the characteristics of the TGEV virulent strains. In vivo, TGEV JS2012 caused 100% mortality in newborn piglets, indicating the strong pathogenicity of this isolate. These results reveal that the JS2012 is a novel natural recombinant TGEV with high virulence. Our findings provide valuable information about genetic diversity and infection mechanism of the coronavirus family.

猪传染性胃肠炎病毒（TGEV）是引起仔猪病毒性肠炎和仔猪腹泻的主要病原之一。本研究从江苏省仔猪粪便中分离到一株 TGEV JS2012 株。系统进化分析显示，TGEV JS2012 株位于 Purdue 和 Miller 两个聚类群之间。重组分析证实，TGEV JS2012 是 Miller M6 和 Purdue 115 之间的自然重组株。与 Miller M6 株相似，毒力型 Purdue 和中国 TS 株在 S 基因上保持遗传完整性和 TGEV 强毒力株的特征。在体内，TGEV JS2012 引起新生仔猪 100%的死亡率，表明该分离株具有很强的致病性。这些结果表明，JS2012 是一株具有高毒力的新型自然重组 TGEV。我们的研究结果为冠状病毒科的遗传多样性和感染机制提供了有价值的信息。