文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

基于CRISPR/Cas的人畜共患病防治策略进展

Advances in CRISPR/Cas-Based Strategies on Zoonosis.

作者信息

Zhang Yi, Peng Qifeng, Zhang Renjun, Li Chao, Xu Quangang, Xia Luming, Wang Youming, Liu Ping, Pan Hong

机构信息

Institute of Zoonosis, College of Public Health, Zunyi Medical University, Zunyi, China.

Key Laboratory of Maternal and Child Health and Exposure Science of Guizhou Higher Education Institutes, Zunyi, China.

出版信息

Transbound Emerg Dis. 2023 Aug 3;2023:9098445. doi: 10.1155/2023/9098445. eCollection 2023.

DOI:10.1155/2023/9098445
PMID:40303816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12017210/
Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) has emerged as the predominant technique for gene editing technique due to its high efficiency and low cost. In the area of zoonosis, CRISPR/Cas is also widely used in different research areas. This paper reviewed the principles of CRISPR/Cas technique and its applications in zoonosis. Moreover, we analyze the shortcomings and weaknesses that currently limit its use, highlight its direction for improvement, and foresee its application prospects in the prevention and treatment of zoonosis. For the purpose of preventing and controlling zoonosis, we need to develop diagnostic method with high sensitivity and specificity, highly protective vaccines, and also better understanding of the pathogenesis. Our review aimed to promote the application and improvement of CRISPR/Cas technique in the above-mentioned areas, and provide brief and comprehensive references for CRISPR/Cas-based research. Through reviewing the advances in CRISPR/Cas-based strategies on zoonosis, we believe that CRISPR/Cas technique will provide more powerful assistance for the prevention and control of zoonosis.

摘要

成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）因其高效性和低成本，已成为基因编辑技术的主要手段。在人畜共患病领域，CRISPR/Cas也广泛应用于不同的研究领域。本文综述了CRISPR/Cas技术的原理及其在人畜共患病中的应用。此外，我们分析了目前限制其应用的缺点和不足，强调了其改进方向，并展望了其在人畜共患病防治中的应用前景。为了预防和控制人畜共患病，我们需要开发高灵敏度和特异性的诊断方法、高保护性疫苗，同时更好地了解其发病机制。我们的综述旨在促进CRISPR/Cas技术在上述领域的应用和改进，并为基于CRISPR/Cas的研究提供简要而全面的参考。通过回顾基于CRISPR/Cas的人畜共患病防治策略的进展，我们相信CRISPR/Cas技术将为人畜共患病的预防和控制提供更有力的帮助。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/5fb8be9ad3a7/TBED2023-9098445.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/40bdf1075e1e/TBED2023-9098445.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/c4c1088e4998/TBED2023-9098445.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/5fb8be9ad3a7/TBED2023-9098445.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/40bdf1075e1e/TBED2023-9098445.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/c4c1088e4998/TBED2023-9098445.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0271/12017210/5fb8be9ad3a7/TBED2023-9098445.003.jpg

相似文献

[1]
Advances in CRISPR/Cas-Based Strategies on Zoonosis.

Transbound Emerg Dis. 2023-8-3

[2]
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas Advancement in Molecular Diagnostics and Signal Readout Approaches.

J Mol Diagn. 2021-11

[3]
Clustered Regularly Interspaced Short Palindromic Repeats and Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein 9 System: Factors Affecting Precision Gene Editing Efficiency and Optimization Strategies.

Hum Gene Ther. 2023-12

[4]
[Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research].

Zhonghua Shao Shang Za Zhi. 2018-4-20

[5]
Genome editing using CRISPR, CAST, and Fanzor systems.

Mol Cells. 2024-7

[6]
CRISPR-Cas systems: ushering in the new genome editing era.

Bioengineered. 2018

[7]
Strategies for the CRISPR-Based Therapeutics.

Trends Pharmacol Sci. 2020-1

[8]
Strategies for Optimization of the Clustered Regularly Interspaced Short Palindromic Repeat-Based Genome Editing System for Enhanced Editing Specificity.

Hum Gene Ther. 2022-4

[9]
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement.

J Zhejiang Univ Sci B. 2021-4-15

[10]
Controlling and enhancing CRISPR systems.

Nat Chem Biol. 2021-1

本文引用的文献

[1]
Current Bioinformatics Tools to Optimize CRISPR/Cas9 Experiments to Reduce Off-Target Effects.

Int J Mol Sci. 2023-3-27

[2]
Prime editing with genuine Cas9 nickases minimizes unwanted indels.

Nat Commun. 2023-3-30

[3]
Identification -like locus from the porcine genome and site-specific integration of recombinase-mediated cassette exchange using CRISPR/Cas9.

Anim Biotechnol. 2023-12

[4]
Genome Editing of Rat.

Methods Mol Biol. 2023

[5]
Generation of APN-chimeric gene-edited pigs by CRISPR/Cas9-mediated knock-in strategy.

Gene. 2023-1-30

[6]
Co-Deletion of A238L and EP402R Genes from a Genotype IX African Swine Fever Virus Results in Partial Attenuation and Protection in Swine.

Viruses. 2022-9-13

[7]
Development and evaluation of a rapid RPA/CRISPR-based detection of .

Front Microbiol. 2022-8-10

[8]
Estimation of the Impact of Foodborne Salmonellosis on Consumer Well-Being in Hungary.

Int J Environ Res Public Health. 2021-9-27

[9]
Comparison of the Feasibility, Efficiency, and Safety of Genome Editing Technologies.

Int J Mol Sci. 2021-9-26

[10]
CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples.

Parasit Vectors. 2021-4-20

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索