Adhikary Kuheli, Kapoor Sukriti, Kotak Sachin
Department of Microbiology and Cell Biology (MCB), Indian Institute of Science (IISc), Bangalore, India.
Molecular, Cell and Developmental Biology, University of California, Los Angeles (UCLA) , Los Angeles, CA, USA.
J Cell Biol. 2025 Jul 7;224(7). doi: 10.1083/jcb.202406059. Epub 2025 Apr 30.
In animal cells, cleavage furrow formation is controlled by localized activation of the GTPase RhoA at the equatorial membrane using cues transmitted from the spindle. Here, we explore the function of LIN-5, a well-studied protein known for its role in aster separation and spindle positioning in cleavage furrow formation. We show that the cortical pool of LIN-5, recruited by GPR-1/2 and important for cortical force generation, regulates cleavage furrow formation independently of its roles in aster separation and spindle positioning. Instead, our data suggest that enrichment of LIN-5/GPR-1/2 at the polar cortical region is essential to ensure the timely accumulation of contractile ring components-myosin II and Anillin at the equatorial cortex. We additionally define a late cytokinesis role of cortical LIN-5/GPR-1/2 in midbody stabilization and abscission. These results indicate that the cortical LIN-5/GPR-1/2 complex contributes to multiple aspects of cytokinesis independently of its roles in spindle positioning and elongation.
在动物细胞中，分裂沟的形成是通过利用从纺锤体传递的信号，在赤道膜处局部激活GTP酶RhoA来控制的。在这里，我们探究了LIN-5的功能，LIN-5是一种经过充分研究的蛋白质，因其在分裂沟形成过程中的星体分离和纺锤体定位作用而闻名。我们发现，由GPR-1/2招募的LIN-5皮质池，对产生皮质力很重要，它独立于其在星体分离和纺锤体定位中的作用来调节分裂沟的形成。相反，我们的数据表明，LIN-5/GPR-1/2在极性皮质区域的富集对于确保收缩环成分——肌球蛋白II和肌动蛋白结合蛋白在赤道皮质及时积累至关重要。我们还确定了皮质LIN-5/GPR-1/2在胞质分裂后期对中间体稳定和脱离所起的作用。这些结果表明，皮质LIN-5/GPR-1/2复合物独立于其在纺锤体定位和延长中的作用，对胞质分裂的多个方面都有贡献。
