解析细菌表面蛋白质组的最新方法

Updated Methods for Elucidating Bacterial Surfaceomes.

作者信息

Jarocki Veronica M, Rezcallah Maria C, Coulon Pauline M L, Padula Matthew P, Djordjevic Steven P

机构信息

Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, Australia.

School of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.

出版信息

Methods Mol Biol. 2025;2908:125-138. doi: 10.1007/978-1-0716-4434-8_9.

DOI:10.1007/978-1-0716-4434-8_9

PMID:40304907

Abstract

The bacterial surfaceome, representing the full complement of surface-expressed proteins, is integral to interactions between bacteria, their hosts, and the environment, influencing processes such as adhesion, invasion, immune evasion, and biofilm formation. These proteins, due to their accessibility and vital roles in bacterial physiology, are prime targets for therapeutic intervention. This protocol describes a detailed methodology for mapping bacterial surfaceomes, including enzymatic surface shaving, cell surface biotinylation, solid-phase extraction, and liquid chromatography-tandem mass spectrometry.

摘要

细菌表面蛋白质组代表了表面表达蛋白的完整集合，对于细菌与其宿主及环境之间的相互作用至关重要，影响着诸如黏附、侵袭、免疫逃避和生物膜形成等过程。由于这些蛋白质易于获取且在细菌生理学中发挥着重要作用，它们是治疗干预的主要靶点。本方案描述了一种用于绘制细菌表面蛋白质组图谱的详细方法，包括酶促表面刮削、细胞表面生物素化、固相萃取和液相色谱-串联质谱分析。