• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用双波长电解质指示剂分析小鼠囊胚滋养外胚层细胞中的Na+浓度模式。

Analysis of Na+ concentration patterns in trophectoderm cells of mouse blastocysts using a dual-wavelength electrolyte indicator.

作者信息

Fujishima Ayaka, Takahashi Kazumasa, Goto Mayumi, Fujishima Akiko, Hirakawa Takeo, Iwasawa Takuya, Shirasawa Hiromitsu, Kumazawa Yukiyo, Terada Yukihiro

机构信息

Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.

出版信息

PLoS One. 2025 Apr 30;20(4):e0322286. doi: 10.1371/journal.pone.0322286. eCollection 2025.

DOI:10.1371/journal.pone.0322286
PMID:40305555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12043133/
Abstract

The developmental process of the mammalian blastocyst involves the intricate interplay of cellular and molecular mechanisms, including electrolyte dynamics within the trophectoderm (TE). We hypothesized that sodium (Na+) is actively transported from the TE into the blastocyst cavity, driving water influx and promoting blastocyst expansion. In this study, we investigated the dynamics of Na+ concentration in the TE of mouse embryos using sodium-binding benzofuran isophthalate (SBFI), a dual-wavelength Na+-sensitive fluorescent indicator. Observations revealed three distinct patterns of Na+ dynamics, each correlating with variations in blastocyst cross-sectional area and developmental outcomes. Embryos exhibiting an initial decrease followed by stabilization of Na+ concentration (Group A) demonstrated the highest rates of hatching, suggesting a relationship between Na+ flux and successful embryonic development. In contrast, embryos with transient increases (Group B) displayed reduced hatching rates and developmental progression. Further, the inhibition of Na+/K+-ATPase activity disrupted Na+ flux and blastocyst cavity expansion, emphasizing its critical role in blastocyst formation. This study highlights the potential of dual-wavelength imaging for elucidating electrolyte dynamics in preimplantation embryos and its implications for optimizing embryo culture systems in reproductive medicine.

摘要

哺乳动物囊胚的发育过程涉及细胞和分子机制的复杂相互作用，包括滋养外胚层（TE）内的电解质动态变化。我们假设钠（Na+）从TE被主动转运到囊胚腔中，驱动水的流入并促进囊胚扩张。在本研究中，我们使用钠结合苯并呋喃异酞酸酯（SBFI），一种双波长Na+敏感荧光指示剂，研究了小鼠胚胎TE中Na+浓度的动态变化。观察结果揭示了三种不同的Na+动态模式，每种模式都与囊胚横截面积和发育结果的变化相关。Na+浓度最初下降随后稳定的胚胎（A组）孵化率最高，表明Na+通量与胚胎成功发育之间存在关联。相比之下，Na+浓度短暂升高的胚胎（B组）孵化率和发育进程降低。此外，抑制Na+/K+-ATP酶活性会破坏Na+通量和囊胚腔扩张，强调了其在囊胚形成中的关键作用。本研究突出了双波长成像在阐明植入前胚胎电解质动态变化及其对生殖医学中优化胚胎培养系统的意义方面的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/48260cce80b7/pone.0322286.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/bee0bb32ac09/pone.0322286.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/b3b14c43aae7/pone.0322286.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/fb9894d59256/pone.0322286.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/5da8086cdb5f/pone.0322286.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/392246bc198b/pone.0322286.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/8a8ea27334c8/pone.0322286.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/6605993a1fa7/pone.0322286.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/c7536587e75e/pone.0322286.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/48260cce80b7/pone.0322286.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/bee0bb32ac09/pone.0322286.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/b3b14c43aae7/pone.0322286.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/fb9894d59256/pone.0322286.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/5da8086cdb5f/pone.0322286.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/392246bc198b/pone.0322286.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/8a8ea27334c8/pone.0322286.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/6605993a1fa7/pone.0322286.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/c7536587e75e/pone.0322286.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0128/12043133/48260cce80b7/pone.0322286.g009.jpg

相似文献

1
Analysis of Na+ concentration patterns in trophectoderm cells of mouse blastocysts using a dual-wavelength electrolyte indicator.使用双波长电解质指示剂分析小鼠囊胚滋养外胚层细胞中的Na+浓度模式。
PLoS One. 2025 Apr 30;20(4):e0322286. doi: 10.1371/journal.pone.0322286. eCollection 2025.
2
Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators.利用电解质指示剂对小鼠胚胎发育过程中的电解质进行实时可视化。
PLoS One. 2021 Jan 29;16(1):e0246337. doi: 10.1371/journal.pone.0246337. eCollection 2021.
3
Na+/K+ ATPase α1 and β3 subunits are localized to the basolateral membrane of trophectoderm cells in human blastocysts.Na+/K+ATPaseα1 和β3 亚基定位于人类囊胚滋养外胚层细胞的基底外侧膜。
Hum Reprod. 2022 Jun 30;37(7):1423-1430. doi: 10.1093/humrep/deac124.
4
Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins.囊胚形成及滋养外胚层紧密连接相关蛋白的正常组装需要钠钾ATP酶β1亚基的表达。
J Biol Chem. 2007 Apr 20;282(16):12127-34. doi: 10.1074/jbc.M700696200. Epub 2007 Feb 22.
5
TEAD4 regulates trophectoderm differentiation upstream of CDX2 in a GATA3-independent manner in the human preimplantation embryo.TEAD4 通过一种 GATA3 非依赖的方式在上游调控人胚胎着床前阶段 CDX2 表达从而调控滋养层分化。
Hum Reprod. 2022 Jul 30;37(8):1760-1773. doi: 10.1093/humrep/deac138.
6
Na/K-ATPase-mediated 86Rb+ uptake and asymmetrical trophectoderm localization of alpha1 and alpha3 Na/K-ATPase isoforms during bovine preattachment development.牛着床前发育过程中钠钾ATP酶介导的⁸⁶Rb⁺摄取以及α1和α3钠钾ATP酶亚型在滋养外胚层的不对称定位
Dev Biol. 1998 May 1;197(1):77-92. doi: 10.1006/dbio.1998.8874.
7
Ouabain stimulates a Na+/K+-ATPase-mediated SFK-activated signalling pathway that regulates tight junction function in the mouse blastocyst.哇巴因刺激 Na+/K+-ATPase 介导的 SFK 激活信号通路，调节小鼠囊胚中的紧密连接功能。
PLoS One. 2011;6(8):e23704. doi: 10.1371/journal.pone.0023704. Epub 2011 Aug 25.
8
The gamma-subunit of the Na-K-ATPase as a potential regulator of apical and basolateral Na+-pump isozymes during development of bovine pre-attachment embryos.钠钾ATP酶的γ亚基作为牛着床前胚胎发育过程中顶端和基底外侧钠泵同工酶的潜在调节因子。
Reproduction. 2002 Sep;124(3):387-97.
9
Tight junctions containing claudin 4 and 6 are essential for blastocyst formation in preimplantation mouse embryos.包含claudin 4和6的紧密连接对于植入前小鼠胚胎中的囊胚形成至关重要。
Dev Biol. 2007 Dec 15;312(2):509-22. doi: 10.1016/j.ydbio.2007.09.049. Epub 2007 Oct 4.
10
Developmental aspects of sodium-dependent transport processes of preimplantation rabbit embryos.植入前兔胚胎钠依赖性转运过程的发育方面
Soc Gen Physiol Ser. 1985;39:211-35.

本文引用的文献

1
Clinical effectiveness and safety of time-lapse imaging systems for embryo incubation and selection in in-vitro fertilisation treatment (TILT): a multicentre, three-parallel-group, double-blind, randomised controlled trial.延时成像系统用于体外受精治疗中胚胎培养和选择的临床效果和安全性（TILT）：一项多中心、三平行组、双盲、随机对照试验。
Lancet. 2024 Jul 20;404(10449):256-265. doi: 10.1016/S0140-6736(24)00816-X.
2
Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators.利用电解质指示剂对小鼠胚胎发育过程中的电解质进行实时可视化。
PLoS One. 2021 Jan 29;16(1):e0246337. doi: 10.1371/journal.pone.0246337. eCollection 2021.
3
Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na-K-ATPase in Mouse Kidney Thick Ascending Limb Tubules.
大麻素的利尿和利钠作用机制：抑制小鼠肾髓质升支粗段管腔侧 Na-K-ATP 酶的证据。
J Pharmacol Exp Ther. 2021 Jan;376(1):1-11. doi: 10.1124/jpet.120.000163. Epub 2020 Oct 21.
4
Human frozen-thawed blastocyst morphokinetics observed using time-lapse cinematography reflects the number of trophectoderm cells.使用延时摄影技术观察到的人类冷冻解冻囊胚形态动力学反映了滋养层细胞的数量。
PLoS One. 2019 Jan 16;14(1):e0210992. doi: 10.1371/journal.pone.0210992. eCollection 2019.
5
Functional Indicators of Glutamate Transport in Single Striatal Astrocytes and the Influence of Kir4.1 in Normal and Huntington Mice.单纹状体星形胶质细胞中谷氨酸转运的功能指标以及Kir4.1在正常和亨廷顿病小鼠中的影响
J Neurosci. 2016 May 4;36(18):4959-75. doi: 10.1523/JNEUROSCI.0316-16.2016.
6
A technique for quantifying intracellular free sodium ion using a microplate reader in combination with sodium-binding benzofuran isophthalate and probenecid in cultured neonatal rat cardiomyocytes.一种在培养的新生大鼠心肌细胞中，结合使用微孔板读数器、钠结合苯并呋喃间苯二甲酸酯和丙磺舒来定量细胞内游离钠离子的技术。
BMC Res Notes. 2013 Dec 26;6:556. doi: 10.1186/1756-0500-6-556.
7
Early cell fate decisions in the mouse embryo.小鼠胚胎中的早期细胞命运决定。
Reproduction. 2013 Mar 1;145(3):R65-80. doi: 10.1530/REP-12-0381.
8
Creation of trophectoderm, the first epithelium, in mouse preimplantation development.小鼠植入前发育过程中滋养外胚层（首个上皮组织）的形成。
Results Probl Cell Differ. 2012;55:165-84. doi: 10.1007/978-3-642-30406-4_9.
9
Ouabain stimulates a Na+/K+-ATPase-mediated SFK-activated signalling pathway that regulates tight junction function in the mouse blastocyst.哇巴因刺激 Na+/K+-ATPase 介导的 SFK 激活信号通路，调节小鼠囊胚中的紧密连接功能。
PLoS One. 2011;6(8):e23704. doi: 10.1371/journal.pone.0023704. Epub 2011 Aug 25.
10
Making the blastocyst: lessons from the mouse.囊胚的形成：来自于小鼠的启示。
J Clin Invest. 2010 Apr;120(4):995-1003. doi: 10.1172/JCI41229. Epub 2010 Apr 1.