Ashoor S H, Knox M J, Olsen J R, Deger D A
J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):693-6.
Reported here is a simple liquid chromatographic (LC) method for the determination of riboflavin in milk (liquid, evaporated, and dry), yogurt, and cheese. The method involves passing liquid samples or filtrates of semisolid and solid samples through a C18 cartridge. Retained riboflavin is then eluted with an aliquot of 50% methanol in 0.02M acetate buffer of pH 4. A volume of the eluate is injected into the LC system consisting of a C18 column, a solvent of water-methanol-acetic acid (65 + 35 + 0.1, v/v) with a flow rate of 1 mL/min, and a UV detector set at 270 nm. The method is precise and accurate and compares favorably with the present AOAC method. Moreover, it involves fewer sample preparation steps and has a total analysis time of less than 1 h.
本文报道了一种简单的液相色谱（LC）方法，用于测定牛奶（液态、浓缩和干燥）、酸奶和奶酪中的核黄素。该方法包括将液体样品或半固体及固体样品的滤液通过C18柱。然后用pH值为4的0.02M醋酸盐缓冲液中的50%甲醇等分试样洗脱保留的核黄素。将一定体积的洗脱液注入由C18柱、水-甲醇-乙酸（65 + 35 + 0.1，v/v）溶剂组成的LC系统中，流速为1 mL/min，并将紫外检测器设置在270 nm。该方法精确、准确，与现行的AOAC方法相比具有优势。此外，它涉及的样品制备步骤较少，总分析时间不到1小时。
