Valenti L P
J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):782-4.
A liquid chromatographic (LC) method is described for the determination of quinine, hydroquinine, sodium saccharin, and sodium benzoate in soft drinks. The method involves simple sample preparation, direct injection onto an octadecylsilane column, and elution with a methanol-acetonitrile-water-acetic acid (20 + 10 + 70 + 1) mobile phase. Eluted constituents are measured spectrophotometrically at 254 nm. The relationship between peak height and concentration was linear between 20 and 120 micrograms/mL for quinine. A relative standard deviation of 0.82% was obtained for commercial samples spiked with quinine, and the average recovery was 100.3%. The proposed procedure is accurate and rapid and can also detect hydroquinine (a natural contaminant of quinine), sodium saccharin, and sodium benzoate. Linear responses ranged from 0.45 to 20 micrograms/mL for hydroquinine, from 54.8 to 219 micrograms/mL for sodium saccharin, and from 10.1 to 145.1 micrograms/mL for sodium benzoate. The reproducibility of the LC method was evaluated with standard solutions of hydroquinine, sodium saccharin, and sodium benzoate, which produced relative standard deviations of 0.42, 0.46, and 1.13%, respectively. The average recoveries for sodium saccharin and sodium benzoate from spiked samples were 99.4 and 100.2%, respectively.
本文描述了一种液相色谱（LC）法，用于测定软饮料中的奎宁、氢奎宁、糖精钠和苯甲酸钠。该方法包括简单的样品制备、直接进样到十八烷基硅烷柱上，并用甲醇 - 乙腈 - 水 - 乙酸（20 + 10 + 70 + 1）流动相洗脱。洗脱成分在254 nm处用分光光度法测定。奎宁的峰高与浓度在20至120微克/毫升之间呈线性关系。加标商业样品的相对标准偏差为0.82%，平均回收率为100.3%。所提出的方法准确、快速，还能检测氢奎宁（奎宁的天然污染物）、糖精钠和苯甲酸钠。氢奎宁的线性响应范围为0.45至20微克/毫升，糖精钠为54.8至219微克/毫升，苯甲酸钠为10.1至145.1微克/毫升。用氢奎宁、糖精钠和苯甲酸钠的标准溶液评估了LC法的重现性，其相对标准偏差分别为0.42%、0.46%和1.13%。加标样品中糖精钠和苯甲酸钠的平均回收率分别为99.4%和100.2%。
