Flynn E J, Cerreta K V, Spector S
Eur J Pharmacol. 1977 Mar 7;42(1):21-9. doi: 10.1016/0014-2999(77)90186-8.
Mice were placed on an immunization schedule known to result in the production of antibodies directed against a variety of barbiturates (antibody binding capacity = 2.71 pmole 3H-phenobarbital bound/ml undiluted serum). The pharmacologic response to barbiturate in these actively immunized mice and suitably treated controls was investigates using rotarod apparatus for monitoring CNS depression. It was found that the response to pentobarbital in actively immunized mice was decreased, as reflected by an increase in the time the mice remained on the rotarod and a shift of the dose--response curve to the right. The decreased pharmacologic response to pentobarbital was not the result of changes in levels of the hepatic drug metabolism components. Furthermore, the alteration of pharmacologic response in actively immunized mice was selective for barbiturate and did not modify the ataxia produced by administration of another depressant agent, ethanol.
将小鼠按照已知可产生针对多种巴比妥酸盐的抗体的免疫程序进行处理（抗体结合能力 = 2.71皮摩尔3H - 苯巴比妥结合/毫升未稀释血清）。使用旋转棒装置监测中枢神经系统抑制，研究这些主动免疫小鼠和适当处理的对照小鼠对巴比妥酸盐的药理反应。结果发现，主动免疫小鼠对戊巴比妥的反应降低，表现为小鼠在旋转棒上停留时间增加以及剂量 - 反应曲线右移。对戊巴比妥药理反应的降低并非肝脏药物代谢成分水平变化的结果。此外，主动免疫小鼠药理反应的改变对巴比妥酸盐具有选择性，并未改变另一种抑制剂乙醇给药所产生的共济失调。
