钙-钙调蛋白-三氟拉嗪复合物的结晶及无钙钙调蛋白结晶的尝试。

Crystallization of calcium-calmodulin-trifluoperazine complex and an attempt at crystallizing calcium-free calmodulin.

作者信息

Kawasaki H, Itoh S, Kasai H, Mitsui Y, Iitaka Y, Nakanishi M, Okuyama T

出版信息

J Biochem. 1985 Jun;97(6):1815-8. doi: 10.1093/oxfordjournals.jbchem.a135242.

DOI:10.1093/oxfordjournals.jbchem.a135242

Abstract

Crystals suitable for X-ray structure analysis were obtained for CaM complexed with both calcium ions and a phenothiazine drug, trifluoperazine (TFP). The TFP/CaM binding ratio in crystals was experimentally determined to be nearly 1. An attempt at crystallizing calcium-free calmodulin (CaM) resulted in rigid but non-birefringent solids which exhibited no X-ray reflections.

摘要

获得了适合进行X射线结构分析的晶体，该晶体是钙离子与一种吩噻嗪药物三氟拉嗪（TFP）结合的钙调蛋白（CaM）。通过实验确定晶体中TFP/CaM的结合比接近1。尝试结晶无钙钙调蛋白（CaM）得到了坚硬但无双折射的固体，这些固体没有X射线反射。