一种用于评估诱导多能干细胞来源的视网膜色素上皮细胞色素沉着的非破坏性图像分析方法。
A Non-destructive, Image Analysis Method for Evaluating Pigmentation in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells.
作者信息
Kumar Gopika S, Chidambaranathan Gowri P, Palakkan Anwar A
机构信息
Immunology & Stem Cell Biology, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India.
出版信息
Methods Mol Biol. 2025;2924:235-248. doi: 10.1007/978-1-0716-4530-7_17.
We have developed an image J macro to analyze pigmentation level in iPSC-derived retinal pigment epithelial cells from bright-field images. Human iPSC-derived retinal pigment epithelial cells were treated with hydroquinone to induce hyperpigmentation, and untreated cells were used as controls for the study. The Image analysis results were validated by estimating melanin spectrophotometrically, after lysing the cells. The developed image analysis method will be useful for screening drugs/compounds that induce hyper/hypo pigmentation in cultured iPSC-derived RPE cells.
我们开发了一种Image J宏程序，用于从明场图像分析诱导多能干细胞（iPSC）来源的视网膜色素上皮细胞中的色素沉着水平。将人iPSC来源的视网膜色素上皮细胞用对苯二酚处理以诱导色素沉着过度，未处理的细胞用作该研究的对照。在裂解细胞后，通过分光光度法估计黑色素来验证图像分析结果。所开发的图像分析方法将有助于筛选在培养的iPSC来源的视网膜色素上皮细胞中诱导色素沉着过度/不足的药物/化合物。
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