He Xiaojun, Wang Qiang, Long Qiang, Zhong Yiming, Qi Zhaoxi, Zhang Yecen, Chang Lan, Qian Bei, Huang Shixing, Wang Xinming, Chen Xuemei, Li Feifei, Yang Xiaomei, Gao Wei Dong, Song Zhizhao, Xu Li, Zhao Qiang
Cardiovascular Medical Center, Department of Cardiovascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Department of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Front Immunol. 2025 Apr 16;16:1538384. doi: 10.3389/fimmu.2025.1538384. eCollection 2025.
The gut microbiota metabolite, short-chain fatty acids (SCFAs), can protect against multiple cardiovascular diseases, while the molecular targets and underlying mechanisms need to be elucidated. One of the primary mechanisms of SCFA benefits was the direct activation of a group of G-protein-coupled receptors (GPCRs), termed free fatty acid receptors (FFARs), the FFAR2 (GPR43), and FFAR3 (GPR41). At present, the distribution of FFAR2/3 in cardiac cells has not been entirely clarified.
Using 18 public single-cell RNA-seq and single-nuclear RNA-seq data of human and mouse hearts, we illustrate the entire atlas of distribution in different regions and cell types in normal and infarcted hearts.
We present the atlas of in the whole human body, normal and infarcted hearts at single-cell resolution. We also illustrated the entire atlas of in normal/ischemic hearts of newborn and adult mice by combining public and newly built sc/snRNA-seq datasets. These findings provide valuable information on the possible effect of SCFAs via FFAR2/3 in the heart and valuable references for future studies.
肠道微生物群代谢产物短链脂肪酸（SCFAs）可预防多种心血管疾病，但其分子靶点和潜在机制仍有待阐明。SCFAs发挥益处的主要机制之一是直接激活一组G蛋白偶联受体（GPCRs），即游离脂肪酸受体（FFARs），其中包括FFAR2（GPR43）和FFAR3（GPR41）。目前，FFAR2/3在心脏细胞中的分布尚未完全明确。
利用18个人类和小鼠心脏的公共单细胞RNA测序（scRNA-seq）和单核RNA测序（snRNA-seq）数据，我们阐明了正常和梗死心脏中不同区域和细胞类型的完整分布图。
我们展示了单细胞分辨率下人体、正常和梗死心脏中FFAR2/3的分布图。我们还通过整合公共和新建的sc/snRNA-seq数据集，阐明了新生和成年小鼠正常/缺血心脏中FFAR2/3的完整分布图。这些发现为SCFAs通过FFAR2/3对心脏可能产生的影响提供了有价值的信息，并为未来的研究提供了有价值的参考。
