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用于叶片脉序网络清理、装片和成像的综合图文操作流程。

A comprehensive illustrated protocol for clearing, mounting, and imaging leaf venation networks.

作者信息

Niewiadomski Isabella, Antonio Monica, Aparecido Luiza Maria T, Boakye Mickey, Carlos Sonoma, Echevarria Andrea, Fontao Adrian, Mann Joseph, Matos Ilaíne Silveira, Salinas Norma, Vu Bradley, Blonder Benjamin Wong

机构信息

Department of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.

School of Biological Sciences University of Utah Salt Lake City Utah USA.

出版信息

Appl Plant Sci. 2025 Mar 7;13(2):e70002. doi: 10.1002/aps3.70002. eCollection 2025 Mar-Apr.

DOI:10.1002/aps3.70002
PMID:40308901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12038745/
Abstract

PREMISE

Leaf venation network architecture can provide insights into plant evolution, ecology, and physiology. Venation networks are typically assessed through histological methods, but existing protocols provide limited guidance on processing large or challenging leaves.

METHODS AND RESULTS

We present an illustrated protocol for visualizing whole leaf venation networks, including sample preparation, clearing, staining, mounting, imaging, and archiving steps. The protocol also includes supply lists, troubleshooting procedures, safety considerations, and examples of successful and unsuccessful outcomes. The protocol is suitable for a wide range of leaf sizes and morphologies and has been used with all major plant groups.

CONCLUSION

We provide a workflow for obtaining high-quality mounts and images of venation networks of a wide range of species, using readily available materials.

摘要

前提

叶脉网络结构能够为植物进化、生态学及生理学研究提供见解。叶脉网络通常通过组织学方法进行评估，但现有方案在处理大型或具有挑战性的叶片时提供的指导有限。

方法与结果

我们展示了一个用于可视化全叶叶脉网络的流程，包括样品制备、透明处理、染色、装片、成像及存档步骤。该流程还包括材料清单、故障排除程序、安全注意事项以及成功和失败结果的示例。该流程适用于各种叶片大小和形态，并且已应用于所有主要植物类群。

结论

我们提供了一种工作流程，使用现成的材料即可获得多种物种高质量的叶脉网络装片和图像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/3d74fe71ed08/APS3-13-e70002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/2ae01803e512/APS3-13-e70002-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/db0ad7d47469/APS3-13-e70002-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/df32b2939567/APS3-13-e70002-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/8e8fa6868498/APS3-13-e70002-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/624d89457231/APS3-13-e70002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/5039fdb5aa83/APS3-13-e70002-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/3d74fe71ed08/APS3-13-e70002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/2ae01803e512/APS3-13-e70002-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/db0ad7d47469/APS3-13-e70002-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/df32b2939567/APS3-13-e70002-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/8e8fa6868498/APS3-13-e70002-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/624d89457231/APS3-13-e70002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/5039fdb5aa83/APS3-13-e70002-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73df/12038745/3d74fe71ed08/APS3-13-e70002-g002.jpg

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