非侵入性微测试技术及应用

Non-invasive micro-test technology and applications.

作者信息

Sun Kai, Liu Yunqi, Pan Yanshu, Di Dongwei, Li Jianfang, Xu Feiyun, Li Li, Mimata Yoshiharu, Chen Yingying, Xie Lixia, Wang Siqi, Qi Wenqian, Tang Yan, Sheng Huachun, Wang Bing, Sun Ruixue, Tan Dingquan, Fu Daohong, Yin Ye, Xue Ao, Shi Yichao, Shao Wenjing, Gong Lei, Jiang Zhijian, Zhang Wei, Wu Qiangsheng, Wang Yaosheng, Lang Minglin, Ye Wenxiu, Xu Weifeng, Wei Shuhe, Shi Weiming, Xu Yue Jeff

机构信息

College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.

Zhongguancun Xuyue NMT Industrial Alliance, Beijing 100080, China.

出版信息

Biophys Rep. 2025 Apr 30;11(2):96-111. doi: 10.52601/bpr.2024.240009.

DOI:10.52601/bpr.2024.240009

PMID:40308937

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12035745/

Abstract

Non-invasive micro-test technology (NMT) reveals dynamic ionic/molecular concentration gradients by measuring fluxes of ions and small molecules in liquid media in 1D, 2D or 3D fashions with sensitivity up to pico- (10) or femto- (10) moles per cm per second. NMT has been applied to study metabolism, signal transduction, genes and/or proteins physiological functions related to transmembrane ionic/molecular activities with live samples under normal conditions or stress. Data on ion and/or molecule homeostasis (IMH) by NMT in biomedical sciences, plant and crop sciences, environmental sciences, marine and space biology as well as traditional Chinese medicine are reviewed.

摘要

非侵入式微测试技术（NMT）通过以一维、二维或三维方式测量液体介质中离子和小分子的通量，揭示动态离子/分子浓度梯度，灵敏度高达每秒每平方厘米皮摩尔（10）或飞摩尔（10）。NMT已被应用于研究在正常条件或应激下，与活样本跨膜离子/分子活动相关的代谢、信号转导、基因和/或蛋白质的生理功能。本文综述了NMT在生物医学科学、植物和作物科学、环境科学、海洋和空间生物学以及传统中医领域中关于离子和/或分子稳态（IMH）的数据。