Cancedda R, Capasso O, Castagnola P, Descalzi-Cancedda F, Quarto N
J Cell Biochem. 1985;28(1):7-14. doi: 10.1002/jcb.240280103.
In cultured chick embryo chondrocytes, type X collagen is preferentially deposited in the extracellular matrix, the ratio between type II and type X collagen being about 5 times higher in the culture medium than in the cell layer. When the newly synthesized collagens deposited in slices from the epiphyseal cartilage of 17-day-old embryo tibiae were isolated, type X collagen was always the major species. In agreement with this result the mRNA for type X collagen was the predominant mRNA species purified from the same tissue. When the total collagen (unlabeled) deposited in the epiphyseal cartilage was analyzed, it was observed that type X collagen represented only 1/15 of the type II collagen recovered in the same preparation. The possible explanations for these differences are discussed.
在培养的鸡胚软骨细胞中，X型胶原蛋白优先沉积于细胞外基质，培养基中II型与X型胶原蛋白的比例比细胞层中的约高5倍。当分离17日龄胚胎胫骨骨骺软骨切片中沉积的新合成胶原蛋白时，X型胶原蛋白总是主要类型。与该结果一致，从同一组织中纯化得到的X型胶原蛋白的mRNA是主要的mRNA类型。当分析骨骺软骨中沉积的总胶原蛋白（未标记）时，发现X型胶原蛋白仅占同一制剂中回收的II型胶原蛋白的1/15。文中讨论了这些差异的可能解释。
