Pébusque M J, Vio-Cigna M, Aldebert B, Seite R
J Cell Sci. 1985 Mar;74:65-74. doi: 10.1242/jcs.74.1.65.
A quantitative stereological analysis was undertaken in nucleoli of rat superior cervical ganglion neurons. In this model, two types of fibrillar centres were observed: (1) small-type fibrillar centres were observed during the light span; and (2) a single large-type fibrillar centre occurred during the dark span near the smaller ones. The present data showed that the drastic increase in the mean volume of fibrillar centres during the dark span involved the occurrence and the progressive enlargement of one single large-type fibrillar centre and a marked rise in the number of small-type centres from 18 +/- 2 to 74 +/- 5 at 1500 h and 0100 h corresponding to light and dark spans, respectively. The total number of these small-type fibrillar centres per nucleolus increased with the total volume whereas their unit volume remained unchanged. This enabled us to establish some relationship with nucleolar organizing regions (NORs).
对大鼠颈上神经节神经元的核仁进行了定量立体分析。在这个模型中,观察到两种类型的纤维中心:(1)在光照期观察到小型纤维中心;(2)在黑暗期,在较小的纤维中心附近出现单个大型纤维中心。目前的数据表明,在黑暗期纤维中心平均体积的急剧增加涉及一个单个大型纤维中心的出现和逐渐扩大,以及小型中心数量的显著增加,在分别对应光照期和黑暗期的1500 h和0100 h时,小型中心数量从18±2增加到74±5。每个核仁中这些小型纤维中心的总数随总体积增加,而它们的单位体积保持不变。这使我们能够建立与核仁组织区(NORs)的一些关系。