核碱基、核苷和核苷酸的微径填充柱阴离子交换液相色谱法。
Microbore packed-column anion-exchange liquid chromatography of nucleobases, nucleosides and nucleotides.
作者信息
Rokushika S, Qiu Z Y, Hatano H
出版信息
J Chromatogr. 1985 Mar 1;320(2):335-42. doi: 10.1016/s0021-9673(01)90511-4.
PMID:4030936
Abstract
Low-capacity anion-exchange resin was packed into a 45 cm X 0.19 mm I.D. fused-silica tubing and applied to micro-column liquid chromatography of nucleobases, nucleosides and isomers of nucleotides. The effects of the chromatographic conditions on the elution behaviour of these compounds were studied. The efficiency of the microbore packed column was comparable with that of the conventional size column.
摘要
将低容量阴离子交换树脂填充到一根45厘米×0.19毫米内径的熔融石英管中，并应用于核碱基、核苷和核苷酸异构体的微柱液相色谱分析。研究了色谱条件对这些化合物洗脱行为的影响。微径填充柱的效率与常规尺寸柱相当。
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