Suzuki Mika, Kimura Tsuyoshi, Hashimoto Yoshihide, Kishida Akio
Laboratory for Biomaterials and Bioengineering, Institute of Science Tokyo, Tokyo, Japan.
Department of Biomedical Engineering, Toyo University, Saitama, Japan.
Front Bioeng Biotechnol. 2025 Apr 16;13:1394714. doi: 10.3389/fbioe.2025.1394714. eCollection 2025.
Decellularized tissue refers to extracellular matrix (ECM) derived from living tissue by removing the cellular components and is used for tissue regeneration. Various decellularized tissue sheets and powders, such as the dermis, urinary bladder matrix, and small intestinal submucosa, have been clinically used as covering and prosthetic materials. Moreover, there is growing interest in the use of decellularized tissue for soft-hard interregional tissue regeneration, including in the ligament-bone, tendon-bone, and periodontal ligament-bone interfaces. The focus in these applications lies in the mechanical properties of the decellularized tissue. Decellularized ligaments and tendons have been developed using various decellularization methods, with a focus on maintaining their shape and mechanical properties, and have been applied orthotopically or ectopically to ligaments and tendons. In the ligament-bone interface, it is suggested that decellularized ligament and tendon are regenerated through the migration and rearrangement of host cells, which is referred to as " tissue regeneration." It is also proposed that decellularized tissue can be used to prepare the complex structure of soft-hard interregional tissue, which consists of an ECM and cell populations with gradual change. In this case, the decellularized soft tissues of ligaments, tendons, pericardium, and others are fabricated and modified with hard tissue components to mimic the gradual structure of soft-hard interregional tissue. In this review, we present a detailed discussion of the regeneration of soft-hard interregional tissue using decellularized tissue.
去细胞组织是指通过去除细胞成分从活组织中获得的细胞外基质（ECM），用于组织再生。各种去细胞组织片和粉末，如真皮、膀胱基质和小肠黏膜下层，已在临床上用作覆盖材料和修复材料。此外，人们越来越关注使用去细胞组织进行软硬跨区域组织再生，包括韧带-骨、肌腱-骨和牙周韧带-骨界面。这些应用的重点在于去细胞组织的力学性能。已经使用各种去细胞方法开发出去细胞韧带和肌腱，重点是保持它们的形状和力学性能，并已原位或异位应用于韧带和肌腱。在韧带-骨界面，有人提出，去细胞韧带和肌腱通过宿主细胞的迁移和重排而再生，这被称为“组织再生”。也有人提出，去细胞组织可用于制备软硬跨区域组织的复杂结构，该结构由ECM和逐渐变化的细胞群体组成。在这种情况下，制造韧带、肌腱、心包等的去细胞软组织，并用硬组织成分进行修饰，以模拟软硬跨区域组织的渐变结构。在这篇综述中，我们详细讨论了使用去细胞组织进行软硬跨区域组织再生的情况。
