Overdiek J W, Hermens W A, Merkus F W
J Chromatogr. 1985 Jun 14;341(2):279-85. doi: 10.1016/s0378-4347(00)84041-4.
A simple and rapid high-performance liquid chromatographic assay is described for the simultaneous determination in serum of the aldosterone antagonist spironolactone and its metabolites 7 alpha-thiomethylspirolactone, 6 beta-hydroxy-7 alpha-thiomethylspirolactone and canrenone. Ultraviolet absorption at 240 nm was used to detect the different compounds after elution on a normal-phase column. Endogenous serum substances did not interfere with the assay. This method provides a convenient tool in pharmacokinetic studies of spironolactone, in contrast to previously reported aspecific fluorimetric assays or time-consuming thin-layer chromatographic analyses of radioactive biological material.
本文描述了一种简单快速的高效液相色谱法，用于同时测定血清中醛固酮拮抗剂螺内酯及其代谢物7α-硫甲基螺内酯、6β-羟基-7α-硫甲基螺内酯和坎利酮。在正相柱上洗脱后，利用240nm处的紫外吸收检测不同化合物。内源性血清物质不干扰该测定。与先前报道的非特异性荧光测定法或对放射性生物材料进行耗时的薄层色谱分析相比，该方法为螺内酯的药代动力学研究提供了一种便捷工具。
