Characterization of gelatin-chitosan films incorporated with Nicotiana tabacum extract nanoliposomes for food packaging applications.

The aim of this innovative research was to develop and assess the characteristics of nanoliposome (NL)-loaded gelatin-chitosan (GL-CS 60:40 (v/v))-based bionanocomposite films incorporating encapsulated Tabacum Nicotiana extract (TE) at concentrations of 5, 10, and 15 % w/v. TE-loaded NLs (TENL) were synthesized using soy-lecithin via the sonication method, resulting in an average size of 110 nm. Observations from Scanning Electron Microscopy (SEM) image and Fourier Transform Infrared Spectroscopy (FTIR) analysis revealed that GL-CS films integrated with TENL exhibited enhanced interconnectivity, a rough surface structure, and the formation of new hydrogen bonds compared to control films. The inclusion of TENL at 5 % and 10 % levels led to notable enhancements in film properties, showcasing improved barrier attributes, thermal stability (63.04 to 68.02), and mechanical strength (407.30 to 802.68 MPa). Moreover, incorporating TENL at 15 % levels enhanced the antioxidant activity of the film from 16 % (control films) to 43 %. The presence of NLs in the films corresponded to heightened antibacterial efficacy against Salmonella enterica and Pseudomonas aerogenosan. This study underscores the potential of GL-CS-loaded TENL edible films as a promising strategy for prolonging the shelf life of perishable food items.