从产单核细胞李氏杆菌中纯化一种产生单核细胞增多的活性物质。
Purification of a monocytosis-producing activity from Listeria monocytogenes.
作者信息
Galsworthy S B, Gurofsky S M, Murray R G
出版信息
Infect Immun. 1977 Feb;15(2):500-9. doi: 10.1128/iai.15.2.500-509.1977.
Abstract
The monocytosis-producing activity (MPA) from Listeria monocytogenes is shown to be associated with the cell envelope. Both MPA and immunosuppressive activity were readily extracted with aqueous solvents and separated as two independent activities by gel filtration and ion-exchange chromatography. As little as 10 microng of the resulting MPA-containing fraction caused a ninefold elevation in the level of circulating monocytes. The molecular weight of the fraction was approximately 22,000, and it contained phosphorus and carbohydrate.
摘要
来自单核细胞增生李斯特菌的产单核细胞增多活性（MPA）被证明与细胞包膜有关。MPA和免疫抑制活性都很容易用水性溶剂提取，并通过凝胶过滤和离子交换色谱法分离为两种独立的活性。所得含MPA的级分低至10微克就能使循环单核细胞水平升高九倍。该级分的分子量约为22,000，并且含有磷和碳水化合物。
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