Nishida M, Kasahara K, Kaneko M, Iwasaki H, Hayashi K
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Jul;37(7):1103-11.
A new human endometrial adenocarcinoma cell line, Ishikawa cells, was established from an endometrial adenocarcinoma from a 39-year-old woman and has been maintained in vitro for more than 3 years. The cells were found to form a monolayer in a mosaic fashion and to tend to pile up. Population doubling time was calculated to be about 36, 29 and 27 hours at the 9th, 40th and 50th generations, respectively. The modal chromosomal number of the cells fell in a diploid range. Histology of the tumor induced in athymic nude mice showed it to be a well differentiated adenocarcinoma which closely resembled the original human tumor. Estrogen receptor and progesterone receptor were demonstrated to occur not only in the induced tumor in athymic nude mice but also in in vitro culture cells. From the fact that the cell growth was maintained in an estrogen-free medium, it appeared that the cells had no estrogen dependency.
一种新的人子宫内膜腺癌细胞系——石川细胞，是从一名39岁女性的子宫内膜腺癌中建立的，已在体外培养超过3年。发现这些细胞以镶嵌方式形成单层，并且倾向于堆积。在第9代、第40代和第50代时，群体倍增时间分别计算为约36、29和27小时。细胞的众数染色体数处于二倍体范围内。无胸腺裸鼠体内诱导肿瘤的组织学检查显示，它是一种高分化腺癌，与原始人类肿瘤极为相似。雌激素受体和孕激素受体不仅在无胸腺裸鼠体内诱导的肿瘤中存在，而且在体外培养细胞中也有表达。从细胞在无雌激素培养基中仍能维持生长这一事实来看，这些细胞似乎没有雌激素依赖性。
