Ekumah John-Nelson, Zhong Mingming, Sun Yufan, Liang Qiufang, Rashid Arif, Virk Muhammad Safiullah, Qayum Abdul, Adade Selorm Yao-Say Solomon, Johnson Nana Adwoa Nkuma, Ren Xiaofeng, Muhammad Awais
Department of Food Science and Engineering, School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 202013, Jiangsu, China; Center for Agribusiness Development and Mechanization in Africa (CADMA AgriSolutions), Ho 00233, Ghana.
Department of Food Science and Engineering, School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 202013, Jiangsu, China.
Int J Biol Macromol. 2025 Jun;311(Pt 1):143709. doi: 10.1016/j.ijbiomac.2025.143709. Epub 2025 Apr 30.
This study presents an approach to addressing the challenges of pronounced syneresis, poor mechanical properties, and rapid digestibility in kudzu starch hydrogel by incorporating rutin (RN) and ferulic acid (FA) at varying concentrations of 1.0 and 2.5 mg/mL. Supplementation improved gel stability as syneresis decreased from 9.4 % (control) to 3.1 % (RN 2.5 mg/mL) and 3.8 % (FA 2.5 mg/mL). This reduction correlated with enhanced phytochemical retention (R = -0.859), indicating that lower syneresis contributed to phytochemical preservation. Structurally, relative crystallinity increasing from 5.21 % (control) to 7.90 % and 8.50 % in RN and FA supplemented gels, respectively complemented by FTIR spectroscopic peak shifts at 3300 cm and 1640 cm, while SEM micrographs displayed a more compact and homogenous structure. The supplemented gels exhibited higher viscosity, elasticity and stability under stress. Resistant starch content also increased significantly from 4.2 % (control) to 12.8 % (RN 2.5 mg/mL) and 14.3 % (FA 2.5 mg/mL), aligning with reduced digestibility. The supplemented gels exhibited enhanced antioxidant activity and antimicrobial inhibition against Escherichia coli and Staphylococcus aureus with high consumer acceptance. These findings establish an approach to developing functional kudzu starch hydrogels with improved stability, bioactivity, and reduced digestibility, while maintaining sensory appeal for gel-based foods as healthier food options.
本研究提出了一种方法,通过加入浓度分别为1.0和2.5mg/mL的芦丁(RN)和阿魏酸(FA)来应对葛根淀粉水凝胶中明显的脱水收缩、较差的机械性能和快速消化性等挑战。添加物提高了凝胶稳定性,脱水收缩率从9.4%(对照)降至3.1%(RN 2.5mg/mL)和3.8%(FA 2.5mg/mL)。这种降低与植物化学物质保留的增强相关(R = -0.859),表明较低的脱水收缩有助于植物化学物质的保存。在结构上,相对结晶度分别从5.21%(对照)增加到RN和FA添加凝胶中的7.90%和8.50%,同时在3300cm和1640cm处的FTIR光谱峰发生位移,而扫描电子显微镜图像显示结构更紧凑且均匀。添加后的凝胶在应力下表现出更高的粘度、弹性和稳定性。抗性淀粉含量也从4.2%(对照)显著增加到12.8%(RN 2.5mg/mL)和14.3%(FA 2.5mg/mL),这与消化率降低相一致。添加后的凝胶表现出增强的抗氧化活性以及对大肠杆菌和金黄色葡萄球菌的抗菌抑制作用,且消费者接受度高。这些发现建立了一种开发功能性葛根淀粉水凝胶的方法,该水凝胶具有更高的稳定性、生物活性和更低的消化率,同时保持了对基于凝胶的食品的感官吸引力,使其成为更健康的食品选择。