Sinurat Ellya, Marraskuranto Endar, Artanti Nina, Zakaria Zainul Amiruddin, Randy Ahmad
Research Center for Marine and Land Bioindustry, National Research and Innovation Agency of Indonesia, Serpong 15310, Indonesia.
Research Center for Marine and Land Bioindustry, National Research and Innovation Agency of Indonesia, Serpong 15310, Indonesia.
Int J Biol Macromol. 2025 Jun;311(Pt 1):143592. doi: 10.1016/j.ijbiomac.2025.143592. Epub 2025 Apr 30.
This study investigated ultrasound extraction (UE) process parameters (time and solvent) to obtain a high yield of fucoidan extracts and associated antioxidant activities from two tropical seaweeds (Sargassum binderi Sonder and Padina australis). UE parameters were time variable (30 and 60 min), solvent variable (distilled water, 2 % CaCl, 0.1 M HCl). Fucoidan's characteristics were evaluated for its total sugar, sulfate content, total phenolic content, and total flavonoid content. Fucoidan extracts were assessed for their antioxidant capabilities by applying 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and Ferric Reducing Antioxidant Power (FRAP) analyses. UE significantly improved the extraction yield in all seaweeds investigated compared to solvent extraction. Using UE, the highest yield was obtained from S. binderi Sonder using HCl for 60 min (22.40 ± 1.48 %), while the lowest yield was obtained from S. binderi Sonder using water for 30 min (6.82 ± 0.21 %). Bioactivities of fucoidan water extract from S. binderi Sonder obtained from UE extraction time of 60 min exhibited the strongest DPPH scavenging activity (% inhibition of 88.42 ± 0.90), ABTS antioxidant activity (% inhibition of 86.64 ± 0.30), and FRAP (% inhibition of 81.96 ± 0.70). UE under optimum conditions proved an efficient, cost-effective, and environmentally sustainable method for recovering physiologically active polyphenols from two species of brown seaweeds.
本研究考察了超声提取（UE）工艺参数（时间和溶剂），以从两种热带海藻（半叶马尾藻和南方团扇藻）中获得高产率的岩藻依聚糖提取物及其相关抗氧化活性。UE参数包括时间变量（30和60分钟）、溶剂变量（蒸馏水、2%氯化钙、0.1M盐酸）。对岩藻依聚糖的总糖、硫酸盐含量、总酚含量和总黄酮含量进行了特性评估。通过1,1-二苯基-2-苦基肼（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和铁还原抗氧化能力（FRAP）分析，评估了岩藻依聚糖提取物的抗氧化能力。与溶剂提取相比，UE显著提高了所有研究海藻的提取产率。使用UE，在60分钟内用盐酸从半叶马尾藻中获得的产率最高（22.40±1.48%），而在30分钟内用水从半叶马尾藻中获得的产率最低（6.82±0.21%）。从UE提取时间为60分钟获得的半叶马尾藻岩藻依聚糖水提取物的生物活性表现出最强的DPPH清除活性（抑制率为88.42±0.90%）、ABTS抗氧化活性（抑制率为86.64±0.30%）和FRAP（抑制率为81.96±0.70%）。在最佳条件下的UE被证明是一种从两种褐藻中回收具有生理活性的多酚的高效、经济且环境可持续的方法。
