Liu Jiaxing, Irigoyen Sonia, Mandadi Kranthi K
Texas A&M AgriLife Research and Extension Center, 2415 East US Highway 83, Weslaco, TX 78596, USA.
Texas A&M AgriLife Research and Extension Center, 2415 East US Highway 83, Weslaco, TX 78596, USA; Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA; Institute for Advancing Health Through Agriculture, Texas A&M AgriLife, College Station, TX 77843, USA.
STAR Protoc. 2025 May 2;6(2):103802. doi: 10.1016/j.xpro.2025.103802.
Identification and functional characterization of candidate effector proteins of pathogens are essential for understanding plant-microbe interactions. Here, we present a protocol for probing candidate effector activity using the Prf-based plant cell death phenotype. We describe steps for co-infiltrating plant leaves with a Prf protein and the effector and visually assess the cell death phenotype as a readout for detecting effector activity. The protocol enables the screening of candidate effectors that suppress programmed cell death by interfering with plant immune responses. For complete details on the use and execution of this protocol, please refer to Wang et al..
鉴定病原体候选效应蛋白并对其进行功能表征对于理解植物与微生物的相互作用至关重要。在此，我们提出了一种利用基于Prf的植物细胞死亡表型来探测候选效应活性的方案。我们描述了将Prf蛋白与效应蛋白共同浸润植物叶片的步骤，并直观评估细胞死亡表型作为检测效应活性的读数。该方案能够筛选通过干扰植物免疫反应来抑制程序性细胞死亡的候选效应蛋白。有关此方案使用和执行的完整详细信息，请参考Wang等人的研究。
