Friede R L, Beuche W
J Neurol Neurosurg Psychiatry. 1985 Aug;48(8):749-56. doi: 10.1136/jnnp.48.8.749.
A computer-assisted method permits collection of large numbers of measurements of fibre profiles in electron micrographs of human sural nerve biopsies. The method is based on simultaneous demonstration of fibre calibre and of sheath thickness in terms of the g-ratio (quotient axon diameter/fibre diameter), and on the recalculation of all parameters for circular fibre profiles. The fibres of small and large diameters of human sural nerves were found to form separate populations with distinctly different trends for sheath thickness and also different patterns of maturation. Preliminary data in neuropathy show that this method gives a better distinction of the extent of damage to either of the two populations; it also permits assessment of the number of regenerating or remyelinating fibres.
一种计算机辅助方法能够在人腓肠神经活检的电子显微照片中收集大量纤维轮廓测量数据。该方法基于以g比值（轴突直径/纤维直径的商）同时显示纤维直径和髓鞘厚度，并对圆形纤维轮廓的所有参数进行重新计算。发现人腓肠神经的小直径和大直径纤维形成了不同的群体，其髓鞘厚度趋势明显不同，成熟模式也不同。神经病变的初步数据表明，该方法能更好地区分这两个群体中任何一个的损伤程度；它还能评估再生或髓鞘再生纤维的数量。
