Chen Yi, Xu Hanyue, Xiao Lirong, Zhang Ming, Yan Naihong
Department of Ophthalmology and Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Department of Ophthalmology and Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Exp Eye Res. 2025 Jul;256:110417. doi: 10.1016/j.exer.2025.110417. Epub 2025 May 2.
Single-cell RNA sequencing (scRNA-seq) has transformed the study of retinal development and diseases by enabling a detailed analysis of cellular diversity within retinal organoids (ROs). ROs generated from pluripotent stem cells mimic the essential characteristics of the human retina and provide a valuable in vitro model for investigating retinal development, cell interactions, and disease mechanisms. This review summarizes the application of scRNA-seq on RO research, emphasizing its capacity to identify distinct cell populations, uncover developmental trajectories, and reveal the molecular signatures of retinal diseases. scRNA-seq provides new insights into retinal neurogenesis, cellular diversity, and the pathophysiology of retinal degenerative diseases. This technology has enabled the identification of novel biomarkers and potential therapeutic targets. Integrating scRNA-seq with other technologies, such as spatial transcriptomics and CRISPR-based screening, can further deepen our understanding of retinal biology and improve treatment strategies.
单细胞RNA测序（scRNA-seq）通过对视网膜类器官（RO）内的细胞多样性进行详细分析，改变了视网膜发育和疾病的研究。多能干细胞产生的RO模拟了人类视网膜的基本特征，并为研究视网膜发育、细胞相互作用和疾病机制提供了有价值的体外模型。本综述总结了scRNA-seq在RO研究中的应用，强调了其识别不同细胞群体、揭示发育轨迹以及揭示视网膜疾病分子特征的能力。scRNA-seq为视网膜神经发生、细胞多样性和视网膜退行性疾病的病理生理学提供了新的见解。这项技术能够识别新的生物标志物和潜在的治疗靶点。将scRNA-seq与其他技术（如空间转录组学和基于CRISPR的筛选）相结合，可以进一步加深我们对视网膜生物学的理解，并改进治疗策略。
