微小膜壳绦虫的亚细胞分级分离，特别涉及标记酶的定位
Subcellular fractionation of Hymenolepis diminuta with special reference to the localization of marker enzymes.
作者信息
Siddiqui A A, Podesta R B
出版信息
J Parasitol. 1985 Aug;71(4):415-21.
A method for subcellular fractionation of Hymenolepis diminuta using whole worm homogenization and differential centrifugation is presented. Different fractions obtained in this study were screened for the presence of enzymes that serve as markers for plasma membrane, brush border, mitochondria, Golgi complex, endoplasmic reticulum, peroxisomes, lysosomes and cytosol. The purity of fractions was also monitored by transmission electron microscopy. The purity of fractions, particularly the brush border membranes, are compared to those obtained by previous methods for H. diminuta or other tissues.
本文介绍了一种使用全虫匀浆和差速离心法对微小膜壳绦虫进行亚细胞分级分离的方法。对本研究中获得的不同级分进行筛选，以检测作为质膜、刷状缘、线粒体、高尔基体、内质网、过氧化物酶体、溶酶体和细胞质溶胶标志物的酶的存在情况。还通过透射电子显微镜监测级分的纯度。将各分级部分，特别是刷状缘膜的纯度，与先前针对微小膜壳绦虫或其他组织所采用方法获得的纯度进行比较。
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