Du Tianhao, Zhou Liangliang, Liu Jia, Wang Xiao, Xie Haoxu, Yang Xu, Yang Yingxin
Department of Rehabilitation Medicine, General Hospital of Northern Theater Command, Shenyang, People's Republic of China.
Liaoning University of traditional Chinese Medicine, Shenyang, People's Republic of China.
Int J Nanomedicine. 2025 Apr 28;20:5509-5527. doi: 10.2147/IJN.S500490. eCollection 2025.
Skeletal muscle injuries are short-term, that occur in people who play sports and train. Regular exercise and sports populations undergo repetitive tearing and regeneration of skeletal muscle, in which muscle damage is a necessary component to produce an oxidative inflammatory response and tissue reconstruction. The primary goals of treating this illness are to reduce the disease process cycle and get rid of symptoms like swelling and inflammation at the site of localized injury. Berberine (BBR) has several pharmacological effects, including anti-inflammatory, anti-tumor, and anti-arrhythmic properties.
In order to treat skeletal muscle injuries, a safe and non-toxic nanogel (BBR/GelMA) was developed for efficient berberine delivery. It also investigated whether BBR/GelMA had anti-inflammatory properties via the NF-κB pathway. Microwave irradiation was added to promote the uptake of BBR in BBR/GelMA by injured skeletal muscle and to accelerate the process of injury recovery.
It turns out that the survival rates of NIH313 and L929 cells decreased to varying degrees in GelMA loaded with different concentrations of BBR, but the survival rates of the two cell lines were the highest at a concentration of 0.125 mg/mL.
In this experiment, the inhibitory effect of BBR/GelMA on inflammation was studied. After NIH-313 and L929 cells were treated with GelMA loaded with different doses of BBR, it was found that the concentration of BBR/0.5 mg/mL had the best inhibitory effect on these two inflammation-inducing cell lines, and this inhibitory effect was related to the drug loading concentration. On the other hand, BBR/GelMA and microwave therapy can play an anti-inflammatory and repairing role in skeletal muscle through NF-κB pathway. In addition, microwave can accelerate the diffusion of BBR in BBR/GelMA within injured skeletal muscle, speeding up the healing process after skeletal muscle injury and shortening the disease cycle.
骨骼肌损伤是短期的，发生在进行运动和训练的人群中。经常锻炼的人和运动员群体的骨骼肌会经历反复的撕裂和再生，其中肌肉损伤是产生氧化炎症反应和组织重建的必要组成部分。治疗这种疾病的主要目标是缩短病程并消除局部损伤部位的肿胀和炎症等症状。黄连素（BBR）具有多种药理作用，包括抗炎、抗肿瘤和抗心律失常特性。
为了治疗骨骼肌损伤，开发了一种安全无毒的纳米凝胶（BBR/GelMA）用于高效递送黄连素。还研究了BBR/GelMA是否通过NF-κB途径具有抗炎特性。添加微波辐射以促进受损骨骼肌对BBR/GelMA中黄连素的摄取，并加速损伤恢复过程。
结果表明，在加载不同浓度BBR的GelMA中，NIH313和L929细胞的存活率不同程度下降，但在浓度为0.125 mg/mL时，两种细胞系的存活率最高。
本实验研究了BBR/GelMA对炎症的抑制作用。用加载不同剂量BBR的GelMA处理NIH-313和L929细胞后，发现BBR/0.5 mg/mL浓度对这两种炎症诱导细胞系的抑制作用最佳，且这种抑制作用与载药浓度有关。另一方面，BBR/GelMA和微波疗法可通过NF-κB途径在骨骼肌中发挥抗炎和修复作用。此外，微波可加速BBR在受损骨骼肌内BBR/GelMA中的扩散，加快骨骼肌损伤后的愈合过程，缩短病程。
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