Le Minh Tam, Nguyen Trung Van, Dang Hong Nhan Thi, Nguyen Quoc Huy Vu
Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.
Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.
Case Rep Obstet Gynecol. 2025 Apr 25;2025:5247242. doi: 10.1155/crog/5247242. eCollection 2025.
Zona-free oocytes (ZFOs) were rarely present when performing the intracytoplasmic sperm injection (ICSI) technique. There have been some reports showing that embryos from these oocytes still result in pregnancy after treatment. These oocytes are often discarded due to quality concerns and difficulties in manipulating and cultivating. This case report shows how ZFOs are handled in in vitro fertilization (IVF). This case report concerns IVF with ICSI and blastocyst culture in an infertile woman with polycystic ovary syndrome. A modified ICSI procedure was proposed to fertilize the ZFO without any damage. The primary outcome measurements involve fertilization assessment and blastocyst development. Among 23 retrieved oocytes, 5 of them were zone ZFOs. Three of the five were fertilized and then developed into good-quality blastocysts. By employing appropriate techniques for fertilization and embryo culture, ZFOs are capable of the development and production of good-quality blastocysts.
在进行卵胞浆内单精子注射（ICSI）技术时，无透明带卵母细胞（ZFOs）很少出现。有一些报告显示，来自这些卵母细胞的胚胎在治疗后仍能导致妊娠。由于质量问题以及操作和培养困难，这些卵母细胞常常被丢弃。本病例报告展示了在体外受精（IVF）中如何处理无透明带卵母细胞。 本病例报告涉及一名患有多囊卵巢综合征的不孕妇女的IVF联合ICSI及囊胚培养。提出了一种改良的ICSI程序，以使无透明带卵母细胞受精而不造成任何损伤。主要结局指标包括受精评估和囊胚发育。在23个回收的卵母细胞中，有5个是无透明带卵母细胞。5个中的3个受精，然后发育成优质囊胚。通过采用适当的受精和胚胎培养技术，无透明带卵母细胞能够发育并产生优质囊胚。
