Österberg Klas, Håkansson Joakim, Mattsson Erney
Department of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden.
Department of Vascular Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden.
J Vasc Res. 2025 May 5:1-8. doi: 10.1159/000546237.
Smooth muscle cells (SMCs) with an origin separate from the local vein wall contribute to formation of intimal hyperplasia (IH) in mouse vein grafts. The recruitment pathway of these cells has not been defined, but circulating progenitor cells and cells from the surrounding tissue or adjacent artery to which the vein graft is anastomosed are potential sources. The aim of this study was to clarify if cells from the adjacent artery contribute to neointimal formation in vein grafts.
Aortic segments from donor SM22α-LacZ mice were anastomosed to vein segments from wild-type (WT) C57BL/6 mice ex vivo followed by implantation of the composite grafts to the right common carotid arteries of WT recipient mice. Six weeks after surgery, the composite grafts were harvested, and histology was analyzed in longitudinal sections. SMCs with origin in the SM22α-LacZ arterial segments were identified with X-gal staining.
LacZ-positive cells were found in the medial layer of the SM22α-LacZ arterial segments but were not found in the IH in the vein graft segment.
SMCs in vein grafts are not recruited from the adjacent artery through migration across the anastomosis.
起源于局部静脉壁之外的平滑肌细胞（SMCs）促成了小鼠静脉移植物内膜增生（IH）的形成。这些细胞的募集途径尚未明确，但循环祖细胞以及来自周围组织或与静脉移植物吻合的相邻动脉的细胞是潜在来源。本研究的目的是阐明来自相邻动脉的细胞是否促成静脉移植物中的新生内膜形成。
将供体SM22α-LacZ小鼠的主动脉段与野生型（WT）C57BL/6小鼠的静脉段在体外进行吻合，随后将复合移植物植入WT受体小鼠的右颈总动脉。术后六周，采集复合移植物，并对纵切片进行组织学分析。通过X-gal染色鉴定起源于SM22α-LacZ动脉段的平滑肌细胞。
在SM22α-LacZ动脉段的中层发现了LacZ阳性细胞，但在静脉移植物段的内膜增生中未发现。
静脉移植物中的平滑肌细胞并非通过跨吻合口迁移从相邻动脉募集而来。
