非编码RNA PROX1-AS1和miR-647：在胃癌中的潜在相互作用及预后价值
Non-coding RNAs PROX1-AS1 and miR-647: Potential Interaction and Prognostic Value in Gastric Cancer.
作者信息
Bure Irina V, Vetchinkina Ekaterina A, Kuznetsova Ekaterina B, Kalinkin Alexey I, Molchanov Artem D, Kiseleva Alevtina E, Alekseeva Ekaterina A, Gorokhovets Neonila V, Rodionov Ivan V, Nemtsova Marina V
机构信息
Laboratory of Medical Genetics, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991, Moscow, Russia.
Research Institute of Molecular and Personalized Medicine, Russian Medical Academy of Continuous Professional Education, 125993 Moscow, Russia.
出版信息
Curr Mol Med. 2025 May 5. doi: 10.2174/0115665240343937250426134726.
BACKGROUND
Gastric cancer (GC) remains one of the most common malignancies and the third cause of cancer-related deaths worldwide. Non-coding RNAs (ncRNAs), including microRNAs and long ncRNAs, can contribute to the pathogenesis and progression of GC and therefore could be its potent diagnostic and prognostic biomarkers. The aim of our work was to estimate the expression of PROX1- AS1 (Prospero Homeobox 1 Antisense RNA 1) and miR-647 (microRNA-647) in GC and investigate their potential interaction and clinical significance.
METHODS
The study included tumor and adjacent non-tumor tissues from 110 GC patients and plasma samples from 65 GC patients; 38 sectional normal gastric tissue samples and 49 plasma samples of healthy donors were included as controls. Expression levels of both ncRNAs were quantified in all samples by using real-time polymerase chain reaction (RT-PCR) and their possible correlations with the clinical and pathological characteristics of patients were analyzed. A potential inverse correlation between PROХ1-AS1 and miR-647 expression was addressed by in vitro experiments in a panel of cancer cell lines.
RESULTS
The expression of PROX1-AS1 and miR-647 was not significantly different in tissues of GC patients and sectional normal gastric tissue samples. However, they have demonstrated a negative correlation both in the tumor and the adjacent nontumor tissue of GC patients. PROX1-AS1 expression was significantly decreased in GC tissues, whereas the miR-647 expression was increased. The expression of the ncRNAs was associated with clinical and pathological characteristics of GC patients. The overexpression of miR-647 led to a significant decrease in PROX1-AS1 expression in five cancer cell lines, including the GC cell line SNU-1.
CONCLUSION
We have demonstrated a negative correlation between PROX1-AS1 and miR-647 in both GC tissues and the cancer cell lines. In addition, expression of both ncRNAs was associated with the primary tumor size. Therefore, these ncRNAs might have potential prognostic value.
背景
胃癌（GC）仍然是全球最常见的恶性肿瘤之一，也是癌症相关死亡的第三大原因。非编码RNA（ncRNAs），包括微小RNA和长链ncRNAs，可促进胃癌的发病机制和进展，因此可能是其有效的诊断和预后生物标志物。我们研究的目的是评估PROX1-AS1（Prospero同源框1反义RNA 1）和miR-647（微小RNA-647）在胃癌中的表达，并研究它们潜在的相互作用和临床意义。
方法
该研究纳入了110例胃癌患者的肿瘤组织和癌旁非肿瘤组织以及65例胃癌患者的血浆样本；38份正常胃组织切片样本和49份健康供体的血浆样本作为对照。通过实时聚合酶链反应（RT-PCR）对所有样本中两种ncRNAs的表达水平进行定量，并分析它们与患者临床和病理特征的可能相关性。通过一组癌细胞系的体外实验探讨了PROХ1-AS1与miR-647表达之间潜在的负相关关系。
结果
PROX1-AS1和miR-647在胃癌患者组织和正常胃组织切片样本中的表达无显著差异。然而，它们在胃癌患者的肿瘤组织和癌旁非肿瘤组织中均呈负相关。PROX1-AS1在胃癌组织中的表达显著降低，而miR-647的表达升高。ncRNAs的表达与胃癌患者的临床和病理特征相关。miR-647的过表达导致包括胃癌细胞系SNU-1在内的五种癌细胞系中PROX1-AS1表达显著降低。
结论
我们已经证明PROX1-AS1和miR-647在胃癌组织和癌细胞系中均呈负相关。此外，两种ncRNAs的表达均与原发肿瘤大小相关。因此，这些ncRNAs可能具有潜在的预后价值。
Zotero 插件