Dantas Leticia Ramos, de Andrade Eduardo Buscarons, Tagliari Giovana Neves, Pereira Giovanna Rosendo, Godoy Luana Czlusniak, Gomes Maria Eduarda Martins, Cachoeira Victoria Camargo, Loureiro Marcelo de Paula, Casagrande Thais Andrade Costa, Loesch Gustavo Henrique, Loesch Maíra de Mayo Oliveira Nogueira, Tuon Felipe Francisco
Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, PR 80215-901, Brazil.
a Departamento de Biotecnologia, Universidade Positivo, Curitiba, Paraná 80020-010, Brazil.
Burns. 2025 Aug;51(6):107522. doi: 10.1016/j.burns.2025.107522. Epub 2025 Apr 30.
Thermal burns are complex injuries that significantly impact morbidity and mortality. This study aimed to evaluate the effectiveness of biomaterials, including collagen gel (CG), collagen sponge (CS), non-decellularized amniotic membrane (NAM), and decellularized amniotic membrane (DAM), in promoting wound healing in a rat burn model.
Sixty Wistar rats were divided into five groups: control, CG, CS, NAM, and DAM. Burn wounds were induced using a heated metal rod, and treatments were applied accordingly. Histological and macroscopic analyses were conducted on days 7 and 14 post-burn to assess inflammation, proliferation of collagen fibers, angiogenesis, and re-epithelialization. Statistical comparisons of wound healing parameters were performed.
By day 7, the CS group showed significant fibroblast proliferation and collagen deposition, facilitating early-stage healing. However, the NAM group demonstrated superior angiogenesis and granulation tissue formation by day 14, with advanced dermal matrix organization and nearly complete epithelialization, including hair regrowth. DAM exhibited moderate proliferation of collagen fibers and angiogenesis but lagged NAM in epidermal regeneration. CG promoted re-epithelialization and vascular remodeling but was less effective than NAM. The control group showed delayed healing with limited repair markers. Overall, NAM was the most effective biomaterial, followed by CG, CS, and DAM.
NAM emerged as the optimal treatment for burn healing, demonstrating superior efficacy in promoting angiogenesis, collagen organization, and epithelial regeneration. These findings underscore the potential of biological membranes in improving burn outcomes. Further human studies are warranted to validate these results and explore clinical applications.
热烧伤是复杂的损伤，对发病率和死亡率有重大影响。本研究旨在评估生物材料，包括胶原凝胶（CG）、胶原海绵（CS）、未脱细胞羊膜（NAM）和脱细胞羊膜（DAM），在大鼠烧伤模型中促进伤口愈合的有效性。
将60只Wistar大鼠分为五组：对照组、CG组、CS组、NAM组和DAM组。使用加热的金属棒诱导烧伤创面，并相应地进行治疗。在烧伤后第7天和第14天进行组织学和宏观分析，以评估炎症、胶原纤维增殖、血管生成和再上皮化。对伤口愈合参数进行统计学比较。
到第7天，CS组显示出明显的成纤维细胞增殖和胶原沉积，促进了早期愈合。然而，NAM组在第14天时显示出更好的血管生成和肉芽组织形成，真皮基质组织更发达，几乎完全上皮化，包括毛发再生。DAM表现出中等程度的胶原纤维增殖和血管生成，但在表皮再生方面落后于NAM。CG促进了再上皮化和血管重塑，但效果不如NAM。对照组愈合延迟，修复标志物有限。总体而言，NAM是最有效的生物材料，其次是CG、CS和DAM。
NAM成为烧伤愈合的最佳治疗方法，在促进血管生成、胶原组织形成和上皮再生方面显示出卓越的疗效。这些发现强调了生物膜在改善烧伤结局方面的潜力。有必要进行进一步的人体研究以验证这些结果并探索临床应用。
