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黄瓜（Cucumis sativus L.）中CsABF/AREB基因家族的全基因组鉴定及表达分析，以及对植物激素和非生物胁迫的响应

Genome-Wide identification and expression analysis of CsABF/AREB gene family in cucumber (Cucumis sativus L.) and in response to phytohormonal and abiotic stresses.

作者信息

Lu Siting, Qiao Yali, Pan Xuejuan, Chen Xinfang, Su Wanyi, Li Ailing, Li Xuelian, Liao Weibiao

机构信息

College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou, 730070, China.

出版信息

Sci Rep. 2025 May 6;15(1):15757. doi: 10.1038/s41598-025-00706-y.

DOI:10.1038/s41598-025-00706-y
PMID:40328839
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12056173/
Abstract

Abscisic acid (ABA)-responsive element binding factors (ABF)/ABA-responsive element binding proteins (AREB)/ABA insensitive protein 5 (ABI5) all belong to the basic leucine zipper (bZIP) transcription factor A subfamily. The bZIP transcription factor family contains 13 subfamilies, namely groups A, B, C, D, E, F, G, H, I, J, K, M and S, and the ABF/AREB/ABI5 gene belongs to A subfamily of the bZIP transcription factor. However, genomic analysis of CsABF/AREB in cucumber (Cucumis sativus L.) has not been systematically studied. In this study, we analyzed the characterization of CsABF/AREB family members and their response to phytohormonal and abiotic stresses. The results showed that a total of 8 genes family members were identified in cucumber. Structural domain analysis showed that the proteins of these family members are highly similar, and all of them belong to the bZIP structural domain. qRT-PCR analysis showed that CsABF/AREB members are expressed in root, stem, and leaf, with the highest expression in root, followed by stem and leaf. In addition, all 8 CsABF/AREB genes respond to ABA and methyl jasmonate (Me-JA). Among them, CsABF7 has the highest expression under both ABA and Me-JA treatments. Drought and salt stress significantly induce CsABF1, CsABF2, CsABF7, and CsABF8 expression. Drought and NaC1 stresses significantly induce the expression of CsABF1, CsABF2, CsABF7, and CsABF8. This study provides a basis for a further understanding of the role of CsABF/AREB homologous genes in response to abiotic stress and lays the foundation for further research on the function of CsABF/AREB.

摘要

脱落酸（ABA）应答元件结合因子（ABF）/ABA应答元件结合蛋白（AREB）/ABA不敏感蛋白5（ABI5）均属于碱性亮氨酸拉链（bZIP）转录因子A亚家族。bZIP转录因子家族包含13个亚家族，即A、B、C、D、E、F、G、H、I、J、K、M和S组，而ABF/AREB/ABI5基因属于bZIP转录因子的A亚家族。然而，尚未对黄瓜（Cucumis sativus L.）中CsABF/AREB进行系统的基因组分析。在本研究中，我们分析了CsABF/AREB家族成员的特征及其对植物激素和非生物胁迫的响应。结果表明，在黄瓜中总共鉴定出8个基因家族成员。结构域分析表明，这些家族成员的蛋白质高度相似，并且它们都属于bZIP结构域。qRT-PCR分析表明，CsABF/AREB成员在根、茎和叶中均有表达，在根中的表达最高，其次是茎和叶。此外，所有8个CsABF/AREB基因均对ABA和茉莉酸甲酯（Me-JA）有响应。其中，CsABF7在ABA和Me-JA处理下的表达均最高。干旱和盐胁迫显著诱导CsABF1、CsABF2、CsABF7和CsABF8的表达。干旱和NaCl胁迫显著诱导CsABF1、CsABF2、CsABF7和CsABF8的表达。本研究为进一步了解CsABF/AREB同源基因在响应非生物胁迫中的作用提供了依据，并为进一步研究CsABF/AREB的功能奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/6b7a5484818e/41598_2025_706_Fig11a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/8332dcd17b1d/41598_2025_706_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/e78b0d0bd464/41598_2025_706_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/6b7a5484818e/41598_2025_706_Fig11a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/8332dcd17b1d/41598_2025_706_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/6d3279a6fa57/41598_2025_706_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/f146e15c1bd7/41598_2025_706_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/1afd32a6349b/41598_2025_706_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/9f279aa72f8d/41598_2025_706_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/2c01108c3f01/41598_2025_706_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/de16b5ff2b1b/41598_2025_706_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/e78b0d0bd464/41598_2025_706_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/c446186e392f/41598_2025_706_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/6eac05eff324/41598_2025_706_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1f/12056173/6b7a5484818e/41598_2025_706_Fig11a_HTML.jpg

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