Niu Shenglian, Li Qiang, Wang Jianling, Li Jiquan, Zhao Yanmei, Xue Hongmei, Ma Li, Zhao Zhijun, Zhang Qingwen
Department of Public Health, Qinghai University Medical College, Xining, Qinghai, 810001, China.
Qinghai Institute for Endemic Disease Prevention and Control, Xining, 810021, Qinghai, China.
Sci Rep. 2025 May 6;15(1):15771. doi: 10.1038/s41598-025-00661-8.
Brucellosis is a significant zoonotic disease that may lead to metabolic profile changes, which remain insufficiently studied. This study utilized an ultra-high performance liquid chromatography coupled with Q Exactive-Orbitrap mass spectrometry (UHPLC-QExactive-Orbitrap MS/MS) to investigate serum samples of acute brucellosis in 32 male patients against 32 well-matched healthy controls. The results revealed nine differential metabolites that correlated with human acute brucellosis, all showing increased levels, except cis-4-hydroxy-D-proline, inosine, hypoxanthine and azelaic acid. These differential metabolites were predominantly involved in metabolic pathways, such as primary bile acid biosynthesis, purine metabolism, taurine and hypotaurine metabolism, and d-amino acid metabolism. This study identified potential metabolite biomarkers of acute brucellosis and laid the foundation for its early diagnosis and prognostic assessment, thus helping to prevent the chronicity of acute brucellosis.
布鲁氏菌病是一种重要的人畜共患病，可能导致代谢谱变化，但对此研究仍不充分。本研究利用超高效液相色谱联用Q Exactive-Orbitrap质谱仪（UHPLC-QExactive-Orbitrap MS/MS），对32例男性急性布鲁氏菌病患者的血清样本与32例匹配良好的健康对照进行了研究。结果显示，与人类急性布鲁氏菌病相关的有9种差异代谢物，除顺式-4-羟基-D-脯氨酸、肌苷、次黄嘌呤和壬二酸外，其余均呈升高水平。这些差异代谢物主要参与初级胆汁酸生物合成、嘌呤代谢、牛磺酸和亚牛磺酸代谢以及D-氨基酸代谢等代谢途径。本研究确定了急性布鲁氏菌病潜在的代谢物生物标志物，为其早期诊断和预后评估奠定了基础，有助于预防急性布鲁氏菌病的慢性化。
