He Yan, Feng Jianing, Shi Wen, Ren Yuerong, Liu Yingyi, Kang Huanmin, Tian Jing, Jie Ying
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmic and Visual Science Key Laboratory, Beijing, China.
Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Northwest University Affiliated People's Hospital, Xi'an, Shaanxi, China.
BMC Ophthalmol. 2025 May 6;25(1):270. doi: 10.1186/s12886-025-04050-7.
To explore the relationship among ocular surface changes, systemic hematologic indexes, and disease activity in primary Sjögren's syndrome patients.
Thirty-three primary Sjögren's syndrome patients and 36 healthy controls were recruited in this cross-sectional study. All participants underwent complete ocular surface testing, including dry eye symptoms and signs, tear multi-cytokine analysis, and conjunctival impression cytology (CIC). Multiple systemic hematologic indexes and disease activity were also evaluated, including autoantibodies, immune cells, the EULAR Sjögren's Syndrome Patient Reported Index (ESSPRI), and the EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI).
Primary Sjögren's syndrome patients exhibited significant dry eye, severe conjunctivochalasis, decreased goblet cell density, and severe squamous epithelial on the ocular surface. Interferon-inducible T cell alpha chemoattractant (I-TAC), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1β, IL-5, IL-8, IL-10, IL-13, IL-21, C-C motif chemokine ligand (CCL)4, interferon-gamma (IFN-γ), CCL20, and tumor necrosis factor-gamma (TNF-α) in the tear fluid of pSS patients changed significantly. Correlation analysis showed that anti-SSA was relevant to ocular surface disease index (OSDI) score, tear break-up time (TBUT), and meibomian gland secretion (MGS). CD8 T cell percentages were relevant to TBUT and corneal fluorescein staining score (CFS). IL-8, IL-13, CCL4, and TNF-α were correlated with RF-IgA. IL-1β, CCL4, and TNF-α were correlated with CD8 T cell counts. IL-5 and CCL20 were correlated with the ratio of helper T cells and suppressor T cells. Tear I-TAC, IL-8, CCL20, and TNF-α were significantly correlated with the ESSDAI of different domains.
Our results revealed that the ocular surface changes in pSS patients were significantly correlated with systemic hematologic indexes and disease activity.
探讨原发性干燥综合征患者眼表改变、全身血液学指标与疾病活动度之间的关系。
本横断面研究纳入了33例原发性干燥综合征患者和36例健康对照者。所有参与者均接受了全面的眼表检查，包括干眼症状和体征、泪液多种细胞因子分析以及结膜印迹细胞学检查（CIC）。还评估了多个全身血液学指标和疾病活动度，包括自身抗体、免疫细胞、欧洲抗风湿病联盟干燥综合征患者报告指数（ESSPRI）和欧洲抗风湿病联盟干燥综合征疾病活动指数（ESSDAI）。
原发性干燥综合征患者表现出明显的干眼、严重的结膜松弛、杯状细胞密度降低以及眼表严重的鳞状上皮化生。原发性干燥综合征患者泪液中的干扰素诱导T细胞α趋化因子（I-TAC）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素（IL）-1β、IL-5、IL-8、IL-10、IL-13、IL-21、C-C基序趋化因子配体（CCL）4、干扰素-γ（IFN-γ）、CCL20和肿瘤坏死因子-γ（TNF-α）有显著变化。相关性分析显示，抗SSA与眼表疾病指数（OSDI）评分、泪膜破裂时间（TBUT）和睑板腺分泌（MGS）相关。CD8 T细胞百分比与TBUT和角膜荧光素染色评分（CFS）相关。IL-8、IL-13、CCL4和TNF-α与RF-IgA相关。IL-1β、CCL4和TNF-α与CD8 T细胞计数相关。IL-5和CCL20与辅助性T细胞和抑制性T细胞的比例相关。泪液I-TAC、IL-8、CCL20和TNF-α与不同领域的ESSDAI显著相关。
我们的结果显示，原发性干燥综合征患者的眼表改变与全身血液学指标和疾病活动度显著相关。
