Demir Derya, Antos Joanna, Švec František, Sklenářová Hana
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
Department of Water Supply and Bioeconomy, Faculty of Environmental Engineering and Energy, Poznan University of Technology, Poznan, Poland.
J Sep Sci. 2025 May;48(5):e70160. doi: 10.1002/jssc.70160.
Deep eutectic solvents (DES) are used as mobile phase and stationary phase modifiers, or as for the stationary phase itself for thin layer/liquid/supercritical chromatography. Their specific properties can improve the separation selectivity, reduce peak tailing, and shorten the separation time. In terms of environmental impact, the advantages of DES are based on their biodegradability, recyclability, and stability in terms of mechanical/chemical/thermal properties. The disadvantages are related to higher viscosity and degradation in aqueous solutions. This review focuses on works that have been published since 2019, the year the excellent comprehensive review by Cai and Qiu was printed. Selected parameters are discussed that should be considered when DES are preferred over commonly used mobile and stationary phases due to green chemistry trends, while taking into account their limitations in modern LC separations. This review also centres on critical aspects of DES applications in the field of liquid and supercritical fluid chromatographic separations.
深共熔溶剂（DES）被用作流动相和固定相改性剂，或用作薄层/液相/超临界色谱的固定相本身。它们的特殊性质可以提高分离选择性、减少峰拖尾并缩短分离时间。在环境影响方面，DES的优势基于其生物降解性、可回收性以及在机械/化学/热性能方面的稳定性。其劣势与较高的粘度以及在水溶液中的降解有关。本综述聚焦于自2019年Cai和Qiu发表优秀的综合综述以来所发表的研究工作。讨论了一些选定的参数，这些参数是在由于绿色化学趋势而优先选择DES而非常用的流动相和固定相时应考虑的，同时考虑到它们在现代液相色谱分离中的局限性。本综述还围绕DES在液相和超临界流体色谱分离领域应用的关键方面展开。
